Spinach Chromatography Lab Report

The bands of color travel the greatest distance are more soluble than the bands that traveled less distance. In this experiment, we did not see chlorophyll a or carotenoids pigments. This could be due to the fact that we used coleus leaves and grass leaves, which do not have a large quantity of carotenoids or chlorophyll a. If we were to use spinach, then we would have seen a different concentration of pigments because the spinach leaf contains certain pigments at a higher

Analysis: The chromatography paper revealed that chlorophyll a, chlorophyll b, carotenes, and xanthophylls, are present in a spinach leaf. This is because a plant needs several different types of pigments in order to photosynthesize properly while simultaneously protecting itself from the dangers of too much sunlight. Chlorophyll a is the primary photosynthetic pigment in all plants, however, all of the other pigments are important as well because they are the ones that provide extra layers of protection in order to shield the plant from harm. Discussion/conclusions: I accept my hypothesis because the data from the lab revealed that a spinach leaf does not only have chlorophyll, although that is all we can see with our naked eye, but also contains carotenes and xanthophylls that help to protect it. One thing I learned through this lab is that chlorophyll, although it is very important to the plant, it is not the only pigment that is important.

Since the some of the sample used for these readings was lost, no additional dilutions were needed. Chlorophyll Determination: The absorbance at 652 nm was measured to determine the total number of chlorophyll a and b in the supernatant. At a wavelength of 652 nm, the two have the same absorbance and carotenoids do not absorb the light. The absorbance was 0.110 A, which was consistent with the standard. The concentration of the total chlorophyll was determined using Beer’s Law: mg/ml.

The plant used in this lab was Phaseolus vulgaris, or the common bean. This plant does C3 photosynthesis. First, my partner and I got two pots with a bean plant in each. In order to get the most accurate and relevant results when compared to one another, the bean plants were relatively the same size. The plants beforehand each had two larger leaves, three smaller leaves, and three leaves in bud form.

Because carbon dioxide is absorbed by the plant during photosynthesis less carbon dioxide present in the chamber is a sign that photosynthesis is working. The four lights used for this experiment range across the light spectrum on both sides in order to test a wider variety of wavelengths. All lights will be placed directly on the spinach leaf at the same distance so as not to give any spinach leaf a different light intensity, which could affect the data. This experiment will be able to show which light, ranging across the light spectrum, will allow the Spinach to perform photosynthesis more efficiently.

Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes.

How did different colors of light affect c02 consumption in spinach leaves? Background Photosynthesis is a process by which plants and other autotrophs make their own food using sunlight energy. Plants take in water, carbon dioxide, and use sunlight energy to create glucose and oxygen. Plants use the glucose as energy and release the oxygen as a by-product.

(From Light Energy to Chemical Energy, Accessed 08 March 2018). Products to are made through photosynthesis as glucose is transported in to the plant as soluble sugars and stored as starch which is insoluble hence it is unable to escape from the cell. Although starch is able to revert back into glucose in order to release energy by respiration both useful for fats and oils for storage through the plant, proteins for growth and repair and cellulose for their cell walls. Figure 2: Structure of a leaf (FTEXPLORING, 2014), Accessed 16 March 2018) Chlorophyll is an essential need to photosynthesis as it is able to trap both blue and red light. It is a complex molecule consisting of three sub types;

photosynthesis and cellular respiration are extremely important in the cycle of energy to sustain life simply because nutrients would not metabolize in a productive manner. Both have numerous stages in which the process of energy occurs, and relationships with organelles located in the eukaryotic cell. Photosynthesis is a process by which green plant and other organism manufacture their food using sunlight to synthesize foods from carbon dioxide and water while cellular respiration is the oxidation of organic compounds that occurs within cells, producing energy for cellular processes. Photosynthesis occurs within organelles called chloroplasts. These organelles can absorb light, and are located inside of leaves.

1. INTRODUCTION Lycopene is natural red color pigment that builds up of photochemical that mostly found in a red colored fruits or vegetables such as tomato, pink guava, watermelon, papaya and pink grapefruits. In 1876, Millardet was first who discovered this red pigment in tomato and later on, Schunck named it as lycopene (Kong, et al., 2010). It belongs to carotenoid group with lack of beta ionic terminal ring. Due to this, lycopene been known as not a pro-vitamin A carotenoid groups which cannot convert lycopene to vitamin A (Kelkel, et al., 2010).

The lab that I did was elodea leaf lab, where me and my partners have to gets a blank glass, take a little leaf out of the water, and put a drop of its water into the glass before putting the lid on. After carefully observed the leaf by using the x65 lens, we puts a drop of salt into the leaf and closed the lid to see how the chloroplasts changed under the microscope. We were basically analyzing the changing of the chloroplasts shape after we puts the salt solution into the elodea leaf. The purpose of our lab was to see how salt solution can affect the elodea leaf cell.

Brassicaceae, is the family that red cabbage belongs to[2]. Red cabbage contains the source of anthocyanins, this anthocyanins contribute the colour to this plant. Since the anthocyanins are unique, by being wisely in colour range by its characteristic which is an indicators. This plant pH-range was very broad compare to others anthocayanins from, e.g. grape skin, elderberry and black current, which only can take a limited degree of colour at pH<4. While anthocyanin in red cabbage can perform

Experiment #7: Column Chromatography of Food Dye Arianne Jan D. Tuozo Mr. Carlos Edward B. Santos October 12, 2015 Abstract Column chromatography is the separation of mixture’s components through a column. Before proceeding with the column chromatography itself, a proper solvent system must be chosen among the different solvents. The green colored food dye is the mixture whose components are separated.

They also contain xanthophyll which makes the leaves look yellow and carotene is what makes the leaves turn orange (Jensen, 2015). During the spring and summer the leaves on trees are green because of photosynthesis occurs. Photosynthesis is the progress of how leaves gets food and water (Jensen, 2015). The chlorophyll gets light from the sun and water from the ground, and then it turns into food and stores it in the leaf (Jensen, 2015).

These compounds might even be able to reverse existing damage (33, 34). Bottom Line: Spinach contains compounds that are essential for vision. These compounds may help block or reverse the damage caused by sunlight. Cancer Prevention