The purpose of this lab is to extract and quantify some major cellular components, which are pigments contained in chloroplasts, DNA, RNA, and proteins. Euglena gracilis is a single-celled eukaryote that has the contains all of these components and so they were the model organism in this experiment. The purpose of this experiment was achieved by utilizing different reactions that are able to extract specific cellular components. The concentrations of these components were measured using absorbance and these values were used to determine the amount of components present in an individual Euglena cell. Additionally, the morphology of the cells was noted. The ratios of the cellular components of the Euglena suspension was compared to the standardized …show more content…
With each reaction, the solution was vortex and centrifuged. The supernatant was collected in a separate tube and the pellet was reacted with 5 ml of acetone. In this experiment, the first supernatant of the three was accidently discarded, therefore only two of the three supernatants were collected for total volume of 10 ml of supernatant A, rather than 15 ml. Using the UltraSpec 3000, an absorbance spectrum was measured at various wavelengths from 400-700 nm (Figure 1). 80% acetone was used as a blank. Since the some of the sample used for these readings was lost, no additional dilutions were needed. Chlorophyll Determination: The absorbance at 652 nm was measured to determine the total number of chlorophyll a and b in the supernatant. At a wavelength of 652 nm, the two have the same absorbance and carotenoids do not absorb the light. The absorbance was 0.110 A, which was consistent with the standard. The concentration of the total chlorophyll was determined using Beer’s Law: mg/ml. Since the concentration of the suspension was approximately 1.8X106 mg/ml, there are approximately: mg of chlorophyll a and b in each cell of the Euglena suspension. Carotenoid
That mixture was then filtered through a coffee filter. Nine test tubes were prepared in order to perform this dye coupled reaction. One contained 5.0ml of the potato and pH buffer mixture, 2.0 ml of hydrogen peroxide, and 1.0 of guaiacol to serve as a blank for the spectrophotometer. Four test tubes were filled with 2.0 ml of hydrogen peroxide and 1.0 ml of guaiacol, used for measurement by the spectrophotometer, each. The last four were filled with 4.0 ml of the potato and pH buffer mixture and 1.0 ml of peroxidase.
Name: Avishak Deb Roy Partners: Leevell Penn, Varugh, Butler Bio 101 Lab Report #1 02.22.2018 Swimming speed of paramecium tetraurelia in different levels of treatment. Introduction Paramecia is a unicellular Protista which are naturally found in aquatic habitats. It is easily cultured in the laboratory. It is oblong shaped and covered with short hairy structure called cilia. Paramecia does not pose any health or ethical concerns and the population can be maintained if there is a food source such as Enterobacter (Biological Foundation 7).
Because carbon dioxide is absorbed by the plant during photosynthesis less carbon dioxide present in the chamber is a sign that photosynthesis is working. The four lights used for this experiment range across the light spectrum on both sides in order to test a wider variety of wavelengths. All lights will be placed directly on the spinach leaf at the same distance so as not to give any spinach leaf a different light intensity, which could affect the data. This experiment will be able to show which light, ranging across the light spectrum, will allow the Spinach to perform photosynthesis more efficiently.
LABORATORY REPORT EXERCISE #5 INTRODUCTION TO THE COMPOUND LIGHT MICROSCOPE, PLANT AND ANIMAL CELLS Name_______________________________Section_____Teacher______________Date________ PRE-LAB QUESTIONS - answer the following questions using your textbook and valid internet sources. Be sure to cite your sources at the end of the prelab. You can type your answers to all questions except #1 and #9 directly into this document and then submit via Canvas. Type the answers for #1 and #9 at the end of the document. 1.
Relebogile Moloko 1155553 Introductory life sciences Assignment 1 Prokaryotes and eukaryotes are two different levels of cells. Prokaryotes are cells that do not have a membrane bound nucleus or organelles sounded by membranes and eukaryotes are cells that have a membrane bound nucleus as well as membrane bound organelle. They have obvious structural differences which result in differences functions and cell efficiency. From my research, I have observed that eukaryotes have structural advantages over prokaryotes.
The living organisms are classified into different groups by means of their differences and similarities. Two of the major/ most important groups are the prokaryotes (Bacteria and Archaea) and eukaryotes (from plants, animals till malaria parasites and fungi). The difference between them is that eukaryotic cells contain membrane-bound organelles, such as the nucleus, whereas the prokaryotic cells don’t. There is also a difference in their cellular structure due to the lack of chloroplast, cell wall and mitochondria in the prokaryotic cells. Furthermore, the DNA material comes in different forms, the DNA of eukaryotic cells comes in forms of chromosomes while the DNA of the prokaryotic cells comes in forms of plasmids (a circular and double-stranded
Introduction: Experiments of the past have confirmed enzyme activity is a significant determinate in the activity of the α-casein with β-casein (Xiaoyu, Yongkang, and Zheng 2012). It’s already been shown before that there are specific enzymes such as Psychromonas ingrahamii that have higher specific activity at lower temperatures. In a separate experiment, it was verified that there was a clear correlation between ortho-Nitrophenyl-β-galactoside (ONPG) concentration and the activity of enzyme beta-galactosidase (β-gal). Higher concentrations of ONPG yielded higher absorbance read by a photospectrometer at 421nm. Since Exercise 1 was conducted under standard atmospheric conditions of a standard classroom setting, further experimentation was profitable to determine whether or not temperature had a significant impact on enzyme activity or not.
Because RuBisCO is so abundantly present in plants it was assumed that the same applies to microalgae. In 1991, J.A. Raven compiled a dataset for RuBisCO in microalgae as the fraction of RuBisCO to total protein by mass, showing values between 2% and 23%. To determine these percentages however, an indirect measurement was performed where RuBisCO is converted on a total protein basis assuming the chlorophyll: total protein mass ratio is 0.0421.
We use Mendelian genetics to study the genetics of C. elegans. C. elegans have very similar genetics structure to humans. C elegans belongs to Phylum Nematode species which is very different from the earthworm. C. elegans is the first eukaryotic organism to have an entire genome sequence. It is very easy and simple to conduct an experiment on C. elegans that’s why the majority of laboratories use this organism.
The objective of this study was to test the phototactic response of Daphnia when exposed to red (>600 nm) and white light. A 30 x 2 cm clear acrylic mesocosms with a 10 cm counting area was filled with distilled water and 10 Daphnia. We counted the number of Daphnia that traveled to the lit counting area after 10 minutes. There were twice as many Daphnia in the lit counting area for the control (white light) compared to the experimental group (red light). The results showed that red light had a negative effect on the phototaxis of Daphnia.
The animal blood cells were examined first. There were three sample tubes, labeled 1-3, set up with 0.1 ml of mammal blood. First, we only added 1.00 ml of Stock 300 mM NaCl to
Slime Molds are among the most dynamic and incredible organisms in the world. There are two distinct types of Slime Molds (Cellular and Plasmodial), the type which forms the basis of this experiment is Plasmodial Slime Molds “Myxogastria” ("Introduction to the "Slime Molds"", n.d.). Plasmodial Slime Molds in a nutshell, are giant single cells abundant in nuclei, which are created when single flagellated cells swarm together and fuse. As a result, one large mass of cytoplasm is formed, which contains numerous diploid nuclei ("Introduction to the "Slime Molds"", n.d.).
The absorbance level @ 520 nm obtained from the spectrometer indicates the amount of urea obtained via measuring the absorbance of the light through the supernatant coloration, which was provided by the
Eukaryotic cells contain a number of organelles that are essential for cell function. Peroxisomes are extremely important function and in multicellular organisms like humans, defects in the peroxisomes can lead to severe disorders like Zellweger syndrome which emphasizes their importance for the functions of both the cells and the organisms (Faust et al., 2014). In mammals, specifically humans, peroxisomes are responsible for a variety of functions, that essential for the functioning of organs, tissues and systems, like fatty acid beta-oxidation and amino acid catabolism (Wander & Waterham, 2006). First of all, peroxisomes are responsible for fatty acid beta-oxidation. Beta-oxidation is the metabolic process in which fatty acids are converted to acetyl-CoA, acyl-CoA, and NADH (Schulz, 1991).
Meiosis Introduction Meiosis is a special type of cell division in which the number of chromosomes in daughter cells is reduced to half, as compared to the parent cell. It takes place in diploid cells only, in animals at the time of gamete production while in plants when spores are produced .There are two meiotic divisions. The first meiotic division is the reduction division whereas the second meiotic division is just like mitosis . Meiosis I