Sargassum latifolium samples were collected during late spring from the intertidal zone of Hurghada, Egypt (27° 12′ N, 33° 50′ E). The macroalgal biomass was air-dried and milled in a home blender. 1.1. Alginate extraction In the extraction process, milled seaweed biomass (1.5 % w/v) was acidified with 2 % citric acid solution with shaking (200 rpm) for 2 h at room temperature. The residual biomass was separated by filtration and washed with distilled water. For alginate extraction, the acidified algal biomass was suspended in 3% Na2CO3 solution at different alkali: alga ratio (20, 40, and 60 mL/g). The different extraction temperatures ranged from 25 to 45º C, and lasted for 1 to 3 h. For each experimental run, sodium alginate was collected by filtration and precipitated with absolute ethanol (1:2 v/v). The mixture was maintained at 4º C overnight. The precipitate was collected by vacuum filtration and allowed to dry at room temperature. The crude alginate yield was calculated from the following equation: Alginate yield (%) = [dry wt. of obtained alginate / dry wt. of sample] × 100 1.2. Block distribution and M/G ratio …show more content…
Alginate sample (30 mg) was hydrolyzed in 10 mL HCl (0.3 M) at 100 ºC for 2 h. After cooling, the mixture was centrifuged (6000 rpm, 45 min), and the supernatant solution was separated and neutralized with 1 M NaOH and referred to as fraction A. The insoluble material was dissolved in 1 M NaOH and the pH was decreased to 2.85 by the addition of 1 M HCl. The suspension was recentrifuged and the supernatant was separated and referred to as fraction B. The insoluble fraction was dissolved by neutralization with 1 M NaOH and referred to as fraction C. The fractions A, B, and C are enriched in MG, MM, and GG blocks
For this lab, zeolite and magnetized zeolite were synthesized and compared with charcoal to find out with would be the most effective in the sequestering of Procion Red dye. Finding the concentration and absorbance of each zeolite, magnetized zeolite, and charcoal, along with a calibration curve, the best adsorbent is determined. Charcoal was the overall best sequestration of the Procion Red dye, since the adsorbent was highest compared to the others. Introduction Pollution has increased in the environment over the years, so the purpose of this experiment is to find the best adsorbent of chemicals to reduce the pollution.
Materials 1. Clean and dry 250 mL beaker. 2. Determine the mass of 2 Alka-Seltzer® tablets. 3.
The percent yield was calculated to be 129.7%. This high percent yield resulted from the presence of
Dalia El-Desoky Organic Chemistry II Lab 05 8 February 2017 Dehydration of 2-methylcyclohexanol Introduction: Dehydration is a common reaction in Organic Chemistry used to produce carbon-carbon double bonds. The dehydration mechanism involves the removal of water from an alcohol to form an alkene. In this experiment, 2-methylcyclohexanol will undergo acid catalyzed dehydration in heat to form three products: 1-methylcyclohexene, 3-methylcyclohexene, and methylenecyclohexane [1]. The reaction is carried out in a Hickman still filled with Drierite, a drying agent composed of CaSO4 which absorbs water.
Cell Respiration Lab Research Question What is the optimal temperature for germinating pea-seeds where the rate of respiration is the greatest? Background Information Cell Respiration refers to the biochemical process conducted by the cells of an organism that combines glucose and oxygen to produce energy in the form of ATP, along with two by-products, water and carbon dioxide. The equation representing this chemical reaction is shown below. C6H12O6 + 6 O2 6 CO2 + 6 H2O
The amount of water the plants need every day for their daily activities such as for growth and photosynthesis is small when compared to what is really lost because of transpiration. Plants have specialized pores called stomata which are in charge for allowing the passing of materials. The stomata pores are surrounded by cells called guard cells whose function is to expand and contract so that they can open and close the stomata. So for example when the plant is losing too much water the guard cells close to stop that.
Doriana Spurrell What different frequencies and types of light would prompt the Spinach leaves to go through the process of photosynthesis effectively? Purpose: The purpose of this experiment was to see which of the four lights that range across the light spectrum would properly and most efficiently help the spinach leaf perform photosynthesis. Background Information: Photosynthesis is the process in which plants use light energy to transform into chemical energy.
Lab Report 5: Acetylsalicylic Acid (Aspirin) Synthesis Name: Divya Mehta Student #: 139006548 Date Conducted: November 19th 2014 Date Submitted: November 26th 2014 Partner’s Name: Kirsten Matthews Lab Section: Wednesday 2:30 L9 IAs Name: Brittany Doerr Procedure: For the procedure, see lab manual (CH110 Lab Manual, Fall 2014) pages 96-98. Wilfrid Laurier University Chemistry Department. Fall 2014. Acetylsalicylic Acid (Aspirin) Synthesis.
The purpose of this experiment is to perform a two step reductive amination using o-vanillin with p-toluidine to synthesize an imine derivative. In this experiment, 0.386 g of o-vanillin and 0.276 g of p-toluidine were mixed into an Erlenmeyer flask. The o-vanillin turned from a green powder to orange layer as it mixed with p-toludine, which was originally a white solid. Ethanol was added as a solvent for this reaction. Sodium borohydride was added in slow portion as the reducing agent, dissolving the precipitate into a yellowish lime solution.
Stoichiometry is a method used in chemistry that involves using relationships between reactants and products in a chemical reaction, to determine a desired quantitative data. The purpose of the lab was to devise a method to determine the percent composition of NaHCO3 in an unknown mixture of compounds NaHCO3 and Na2CO. Heating the mixture of these two compounds will cause a decomposition reaction. Solid NaHCO3 chemically decomposes into gaseous carbon dioxide and water, via the following reaction: 2NaHCO3(s) Na2CO3(s) + H2O(g) + CO2(g). The decomposition reaction was performed in a crucible and heated with a Bunsen burner.
(150.22g/mol)(3.5 x 10^-3 mol of nucleophile) = 0.525 g Actual yield = 0.441 g, Percent Yield = (0.441g/0.525g) x 100% = 84% 10. Percent recovery from recrystallization = (0.172g/0.441g) x 100% = 38% 11.
Jaspreet Singh Professor Paratore Biology 1 November 1, 2014 Spectrophotometry Identifying Solutes and Determining Their Concentration Statement of the Exercise or of the Problem The purpose of the lab experiment was to attain the following objectives: • Learning to Operate the Spectrophotometer • Construct absorption spectra for cobalt chloride and chlorophyll. Hypothesis If greater and higher concentrations of cobalt chloride are added to each solution then greater amounts of light would be absorbed by each solution. Thus a liner relationship will result in which the absorbance of a substance would be proportional to its concentration, which will be depicted, in a linear graph.
Luminol is a chemical that forensic scientists use to find traces of blood at crime scenes. Before application, a crime scene investigator will assemble the solution by combining luminol, sodium hydroxide, and hydrogen peroxide. The sodium hydroxide and hydrogen peroxide are what initially sets off the chemical reaction with the luminol. Then the investigator sprays it over the crime scene. If there is any blood present, the iron in it will act as a catalyst in the chemical reaction.
The final product weight for percent yield was only the solid E product, which missed one half of the final product produce. If both products were weight, the percent yield would have been larger that it was. Instead of 22.33%, it could have been 44.66%. To prove that both products were obtained, but only one of the two products was analyze, a TLC plate of the DCM layer, that contains both products, and of the final product, was obtain.
Algae can capture the CO2 so reduce global warming and it can be used for production of biofuels and some other secondary metabolites such as biodiesel, bioethanol, bio hydrogenetc. (Loubiere .et.al. 2009). 1 kg of algae can utilizes 1.83 kg of carbon (Brennan and owende, 2010). The problems with algae are the temperature of flue gases is high and presence of impurities like NOx, SOx and other impurities of fossil fuels. Changes in temperature and presence of light and other seasonal variation are the major problems in open systems (Brennan and Owende, 2010).