Red Dye Concentration Experiment

For this lab, zeolite and magnetized zeolite were synthesized and compared with charcoal to find out with would be the most effective in the sequestering of Procion Red dye. Finding the concentration and absorbance of each zeolite, magnetized zeolite, and charcoal, along with a calibration curve, the best adsorbent is determined. Charcoal was the overall best sequestration of the Procion Red dye, since the adsorbent was highest compared to the others. Introduction Pollution has increased in the environment over the years, so the purpose of this experiment is to find the best adsorbent of chemicals to reduce the pollution.

Something that was interesting to find out though, in the description of the reagent color test they show the lighter color on top and the darker one on the bottom. But just as the test that was conducted showed and what Officer had said was that the darker color is always on the top and the lighter on

ABSTRACT To catalyze a reaction, an enzyme will grab on (bind) to one or more reactant molecules. In this experiment we examined how increasing the volume of the extract added to the reaction would affect the rate of the reaction. The enzyme used was horseradish peroxidase which helps catalyze hydrogen peroxide. Using different pH levels, the absorbance rate of the reaction was measured to see at which condition the enzyme worked best. The rates of absorption were calculated using a spectrophotometer in 20 second intervals up to 120 seconds.

Red Bead Experiment Submitted By: Sarabjeet Singh (C0712487) Arshdeep Singh (C0719013) Harshpreet Singh (C0713155) Lovish (C0717943) Introduction Red and white beads experiment explained in video mainly based on the Integrated Environment, Safety and Health Management System, it includes all the all the core functions of ISMS environmental management aspects in all five core management functions that support work planning or work scope, hazard analysis, hazard control, work performance, and feedback and improvement. The instructor of the Red Bead experiment, Dr. Deming started with the definition of the work scope, the scope definition serves the purpose of stating exactly what it is that an organization does that is certified to be effectively controlled by the requirements of the standard. Dr. Deming gave different examples by the red and white beads.

3mL of the liquid in each of the vials were added into cuvettes and measured in the spectrophotometer. Before each time point the photo spectrometer was zeroed using a cuvette with 3mL of distilled water. If any of the results were considered unusual the machine was zeroed again and the sample was retested. The results from the spectrophotometer test were recorded in a table. The experiment was repeated six times to gain a sample size of six.

Record the amount of absorbance by converting transmittance every 5 minutes for a total of 20 minutes. Repeat all of these steps for the cantaloupe, banana, replacing the blank each time to recalibrate the spectrophotometer. After recording all the percent transmittance value, the data was then converted into absorbance value by using the absorbance conversion table. The information was placed on a plotted graph

Hypothesis: Increasing substrate concentration will increase the initial reaction rate until it stops increasing and flattens out. Independent Variable: Substrate concentration Dependent Variable: The substrate itself, 1.0% Hydrogen Peroxide How Dependent Variable will be Measured: Hydrogen Peroxide will be used in every experiment, just with different test tubes. The amount of Hydrogen Peroxide in the mixing table is the amount that will be added to each test tube.

“In 1775, more than a million pounds of indigo was exported from South Carolina to England” (Indigo’s Political, Economic, Cultural History 1). This massive amount of dye being traded was due to many factors that made it nearly the perfect trade commodity. The process of indigo dye being made by slaves in South Carolina in the 1700s is shown very well in the book Someone Knows My Name by Lawrence Hill. The production of this indigo dye has an extensive history of being extremely arduous to produce naturally, but results in a compact substance that will last a long period of time and be very valuable.

“Who am I?” This question has been swimming in Red’s mind until he discovers who he really is. Red: A Crayon’s Story revolves around a blue crayon who is supposed to be red; it says so on his label. However, every time he tried to draw red objects like strawberries, ants, and fire trucks, they turn out blue. The story is written from a pencil’s perspective, in which the said pencil is Red’s teacher.

PRACTICAL 4 Materials and Methods Measurement of DNA concentration The most common technique to measure DNA concentration is measurement of absorbance. We had used 1:20 dilution of the DNA sample and the reading was expected to be in the range of 0.1-1.5 OD260. 5µl of DNA and 95µl of PBS buffer were mixed together and inserted into a clean cuvette. Then it was put inside the spectrophotometer.

From the moment you awaken to the moment of you fall asleep your eyes help your brain collect the images of the world. From different structures to sizes, difference in distance, variations, and most intriguing, color. Color is the reflection of light from an object or source. But how is color gathered and interpreted by our brains? We are able to see objects by the reflection of light an object emits which passes through different structures in our eyes.

Use these results to determine the product concentration, using Beer-Lambert’s Law: A= ɛCl (where A is the absorbance, ɛ is the molar absorptivity, C is the product concentration and l is the length of solution that the light passes through). Calculate the product concentrations at every minute for 10 minutes for all 7 of the test tubes using Beer-Lambert’s Law. Plot a graph of product concentration vs. time and then use the gradients of the 7 test tubes to determine the velocities of the reaction. After calculating the velocities, plot a Michaelis-Menten graph of velocity vs. substrate concentration.

The 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted of 0.1ml, 0.2 ml, and 0.4 ml of hydrogen peroxide. In a separate tube, the control was made up of turnip extract and guaiacol, known as the color reagent. This was recorded the absorbance every 20 seconds for 3 minutes.

Practical I: Acid-base equilibrium & pH of solutions Aims/Objectives: 1. To determine the pH range where the indicator changes colour. 2. To identify the suitable indicators for different titrations. 3.

The ammonia: 1-butanol (1:1) solvent was the appropriate solvent to use for the column chromatography of food dye because it exhibited the properties of a good solvent system. A total 8 colored eluents were collected. The eluents had colors of pink, dark red, dark blue, dark green, light green, yellow, orange and light yellow respectively and