Alkaline Phosphatase Enzyme Lab Report

Title: Enzymes Abstract: Enzymes can catalyze chemical reactions by speeding up the chemicals activation energy. Temperature and pH are just two of the factors that affects enzymes and their involvement with chemicals and the way they function. Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. The following information consist of the recordings of when it was exposed to four different pH levels to come up with an optimum pH and IRV at the end. Introduction: Enzymes are proteins that are used in reactions in living organisms.

It was hypothesized that the optimal pH for the enzyme was pH 7 while the 1.0 ml peroxidase would have the best reaction rate. At the end of the experiment the results prove the hypothesis to be incorrect. INTRODUCTION Enzymes are proteins that allow a reaction to speed up. These proteins are made up of monomers known as amino acids.

Objective Bio160 Lab 5: Enzyme Activity May 7, 2015 The objective of this experiment was to note the effect of temperature effects on enzyme rates of reaction. Enzymes are macromolecules that make up significant portions of living organisms. They are made up of repeating subunits of monomers that are referred to as polymers.

Although it was expected for water to be the optimal pH, it was also assumed that more drastic activity would happen with the other pH’s. For example, it was thought that it would still have some noticeable increase; however, when looking at the data and the graph, the numbers oscillate with no noticeable positive or negative trend. Tables 1 and 2 show that the absorbance rate in comparison to the absorbance rate in Table 3 are significantly smaller. Furthermore, after calculating the processed data for reaction rates and looking at the graph, pH 7 water had the highest rate. This experiment gives a good insight for future references about enzymes and the effect of environmental factors and its functions.

The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.

Hypothesis: Increasing substrate concentration will increase the initial reaction rate until it stops increasing and flattens out. Independent Variable: Substrate concentration Dependent Variable: The substrate itself, 1.0% Hydrogen Peroxide How Dependent Variable will be Measured: Hydrogen Peroxide will be used in every experiment, just with different test tubes. The amount of Hydrogen Peroxide in the mixing table is the amount that will be added to each test tube.

purpose the propose of this experiment was too see if the chemical reaction of a enzyme can be made faster. Hypothesis I think that a warm environment would be best to make an enzyme’s reaction faster. because a protein can move faster in heat.

LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.

TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 99.05 99.12 98.71 99.57 98.65 101.98 100.75 TRIAL 2 97.89 96.32 95.89 98.01 97.99 97.83 95.42 TRIAL 3 99.38 96.19 91.58 96.53 97.09 97.11 99.31 ENZYME CONCENTRATION: 2X ENZYME (ABSORBANCE AU) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 0.055 0.132 0.198 0.249 0.289 0.321 0.347 TRIAL 2 0.073 0.145 0.202 0.246 0.281 0.311 0.337 TRIAL 3 0.063 0.212 0.321 0.399 0.457 0.502 0.536 ENZYME CONCENTRATION: 2X ENZYME (TRANSMITTANCE %T) TRIALS 0 SECS 20 SECS 40 SECS 60 SECS 80 SECS 100 SECS 120 SECS TRIAL 1 88.19 73.8 63.42 56.37 51.43 47.79 44.94 TRIAL 2 84.61 71.57 62.86 56.74 52.36 48.84 45.99 TRIAL 3 86.56 61.43 46.72 39.93 34.9 41.51 29.12 ENZYME CONCENTRATION:

LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.

Stoichiometry of a Double Displacement Reaction The objective of this lab is to find the percent yield of a product of a double displacement reaction. Procedure: Refer to handout entitled “Stoichiometry of a Double Displacement Reaction” Materials: Refer to handout entitled “Stoichiometry of a Double Displacement Reaction” Data & Observations: Data Table Calculated Molar Mass of CuSO4•5H2O 249.677 g Calculated Molar Mass of CuO 79.545 g Starting mass of CuSO4•5H2O 2.050 g Mass of 100-ml beaker and filter paper 52.600 g Mass of 100-ml beaker, filter paper, and CuO precipitate 53.450 g Calculations:

Enzymes speed up chemical reactions enabling more products to be formed within a shorter span of time. Enzymes are fragile and easily disrupted by heat or other mild treatment. Studying the effect of temperature and substrate concentration on enzyme concentration allows better understanding of optimum conditions which enzymes can function. An example of an enzyme catalyzed reaction is enzymatic hydrolysis of an artificial substrate, o-Nitrophenylgalactoside (ONPG) used in place of lactose. Upon hydrolysis by B-galactosidase, a yellow colored compound o-Nitrophenol (ONP) is formed.

Errors that could have occurred in this experiment are cross contamination. Although steps had been taken to minimize this test tubes were not completely sterilized between different experiments. In regards to the boiling enzyme the enzyme may not have been in the boiling water long enough to produce the results expected. The hypothesis is partially supported, when the enzymes concentration is altered the rate of reaction correlates to the the concentration. The hypothesis however is not supported when discussing how boiling the enzyme effects the rate of reaction.

ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.

The gradient gave the value of K, the rate constant for the reaction. Figure 2 shows the plotted graph Figure 2. From the