Decomposition of Aspirin Studied with UV/Visible Absorption Spectroscopy Aims: To determine the concentration of salicylic acid, formed from the hydrolysis of Aspirin, at regular intervals using the UV/Visible Absorption Spectroscopy From the concentration of salicylic acid, concentration of Aspirin to be determined using an equation Calculate the rate constant of this reaction and its order from a plot of graph of ln(aspirin) vs time Discuss the overall flaws and improvements to the experiment Results: As per schedule1, 0.212g of aspirin was added to 50 ml boiling water to form salicylic acid in a 100 ml flask, of which 1 ml was then pipetted to a 50 ml volumetric flask at the 5th min. Following an ice bath, the solution was mixed …show more content…
Upon finding the actual concentrations of salicylic acid, concentration of aspirin in the flask at various times can be found using the equation [aspirin]t = [aspirin]0 – [salicylic acid], since at constant volume, number of moles of initial aspirin decrease to form salicylic acid. Initial concentration of aspirin formed as follows: [aspirin]0 = 0.212g / (180.157gmol-1 * 50/1000 L) = 0.0235 mol L-1. Thus using the first test as sample, [aspirin]t = 0.0235 – 9.981*10-4 = 0.0225 mol L-1. To find the rate constant, we will need to log the value of [aspirin]t and plot it against time to find the rate constant. Figure 1 shows the diluted and actual concentrations of salicylic acid, the concentration and log value of aspirin at various times. Time / min C1 / mol L-1 C2 (actual) / mol L-1 [Aspirin] / mol L-1 ln([Aspirin]) 0 1.996*10-5 9.98*10-4 0.0225 -3.79 10th 6.925*10-5 3.46*10-3 0.0200 -3.91 20th 1.135*10-4 5.68*10-3 0.0178 -4.03 30th 1.372*10-4 6.86*10-3 0.0166 -4.10 40th 1.653*10-4 8.27*10-3 0.0152 -4.18 50th 1.828*10-4 9.14*10-3 0.0144 -4.24 60th 1.953*10-4 9.77*10-3 0.0137 -4.29 Figure 1. A graph of ln([aspirin]t) against time (min) was plotted. The gradient gave the value of K, the rate constant for the reaction. Figure 2 shows the plotted graph Figure 2. From the …show more content…
The hydrolysis formed salicylic concentration which was mixed with iron(III) solution to form a purple complex. This was then use to study under the UV/Visible absorption spectroscopy which gave absorbance values recorded at 525nm to determine the concentration of salicylic acid using the Beer Lambert’s Law and later corrected to find the actual concentrations. The concentrations of aspirin at various intervals was found from salicylic concentrations. Upon plotting a graph of ln(aspirin) vs time, it produced a linear equation from which the gradient gave the rate constant of 0.0083min-1 and the overall shape of the graph concluding this reaction to be pseudo first order with respect to the concentration of aspirin with the deviations and improvements as
When each drop of chemical was carefully squeezed onto the surface of the lab table, the time of evaporation was timed carefully, capturing the exact times each of the substances completely finished evaporation. As a result of this, Acetone evaporated instantly, within just less than a minute, 57 seconds. Propanol and Acetic Acid followed a while afterward, with Propanol evaporating in 8 minutes and 40 seconds and Acetic Acid in 30 minutes and 43 seconds. The distinct and differing times of evaporation of all three chemicals represented the differences in the intermolecular forces that each of the chemicals possesses within their molecules and how they cause each substance to behave when left out to evaporate. Weaker intermolecular forces do not take long to fully evaporate because of the lack of energy required to weaken their bonds, hence Acetone takes less time to evaporate compared to Propanol and Acetic
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
The Identity of the unknown, in this case unknown A, was determined to be acetyl salicylic acid. This was determined by comparing the melting points of the purified unknown crystals to the crude unknown. The known melting point range for acetyl salicylic acid is 135°C -136°C. The Unknown’s melting point was slightly off at 128.6°C - 132°C. This can be due to impurities of the crystals during the purification process.
The topic that the scientist has researched is the reaction rate of different particle sizes. In the experiment, the scientist will discover how the particle size of Alka Seltzer affects the rate of chemical reaction with water. The independent variable in the experiment is the particle size of the Alka Seltzer, while the dependent variable is the rate of reaction, or the volume of Carbon dioxide. The volume of carbon dioxide will be measured in ml. Also, a few of the constants in the experiment will be the amount of water, and amount of tablets.
Lab Report 5: Acetylsalicylic Acid (Aspirin) Synthesis Name: Divya Mehta Student #: 139006548 Date Conducted: November 19th 2014 Date Submitted: November 26th 2014 Partner’s Name: Kirsten Matthews Lab Section: Wednesday 2:30 L9 IAs Name: Brittany Doerr Procedure: For the procedure, see lab manual (CH110 Lab Manual, Fall 2014) pages 96-98. Wilfrid Laurier University Chemistry Department. Fall 2014. Acetylsalicylic Acid (Aspirin) Synthesis.
Later on the color changed to green, which indicated the pH was 8.0. Then when we tested the pH with the pH strips they both showed the pH as being 5.0. After that, we added HCl or stomach acid and both drugs dissolved and were soluble. We tested the pH and it dropped to 1.0. They both turned a brownish-yellow when the iron nitrate was added and no other pain reliever or antacid looked like those two.
Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes.
Aspirin is considered a “polydrug” due to its variety of uses stretching from pain-relief to disease prevention. Salicylic acid is derived from the bark and leaves of the willow tree. Salicylic acid belongs to a group of phytochemicals which have been shown to have positive effects on human health. Salicylic acid is a phenolic compound that can be found in a variety of plants and is a crystal organic carboxylic acid. However, it is more commonly viewed as the primary metabolite and active compound of acetyl salicylic acid, which has been used as an anti-inflammatory drug by physicians for over 100 years.
Also, the capillary tube may have been contaminated. This lead to nearly identical marking on the TLC plate and similar Rf values. Due to this, it is not possible to conclude whether or not acetaminophen was separated from the sample of Excedrin. The Rf value of the pure isolated caffeine was 0.28. This was 0.16 higher than the Rf value of the pure caffeine.
TLC was used to identify the actual unknown product as well as other products/reactants present in the filtered solution. The procedure was conducted by placing a TLC plate in a developing chamber that is filled with a small amount of solvent. The solvent cannot be too polar because it will cause spotted compounds on the TLC plate to rise up too fast, while a very non-polar solvent will not allow the spots to move. The polarity of the spots also determines how far it moves on the plate; non-polar spots are higher than polar ones. After spots on the TLC form, the Rf values are calculated and used to analyze the similarity of the compounds.
The regression coefficient value of zero order kinetic plot was found to be 0.974 and the slope was found to be 3.862 (figure 14). Figure 14: Zero order plot for Optimized formula First order kinetics plot: Here the graph is plotted between log cumulative percent drug remaining Vs time. Regression coefficient is calculated and interpreted. The regression coefficient value of this plot was found to be 0.955 and the slope was found to be 0.046 (figure15).
Practical I: Acid-base equilibrium & pH of solutions Aims/Objectives: 1. To determine the pH range where the indicator changes colour. 2. To identify the suitable indicators for different titrations. 3.
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
It was calculated and found that the concentration of benzoic acid was higher at 30℃ (0.0308M) than at 20℃
2.0 1.8 1.5 1.5 Trial 4 2.5 2.3 1.7 1.6 1.5 Trial 5 1.9 1.8 1.6 1.6 1.4 Average 2.1 1.94 1.72 1.54 1.52 Analysis The results of Table 1 are shown graphically in Figure 2 where the calculated and measured Iodine Solution are plotted with respect to the amount of days. The graph can be observed, showing that the measured value of the iodine is very close to the theoretical one. It can be seen that each time the Vitamin C is frozen, the iodine solution reacting with the Acid decreases. This means that the Ascorbic Acid is being destroyed every time it is frozen.