How Does The Concentration Of An Enzyme Affect The Digestion Rate Of The Substrate

Cofactor- Molecules that aren’t proteins nor organic, but help make the reaction go faster when they connect to the active site. 9. competitive inhibitor- prohibits the reaction from taking place by going into the enzyme’s active site so the substrate can’t. 10.

Title: Enzymes Abstract: Enzymes can catalyze chemical reactions by speeding up the chemicals activation energy. Temperature and pH are just two of the factors that affects enzymes and their involvement with chemicals and the way they function. Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. The following information consist of the recordings of when it was exposed to four different pH levels to come up with an optimum pH and IRV at the end. Introduction: Enzymes are proteins that are used in reactions in living organisms.

The enzymeʼs have an active site that allows only certain substances to bind, they do this by having an enzyme and substrate that fit together perfectly. If the enzyme shape is changed then the binding

The products are released from the enzyme surface to regenerate the enzyme for another reaction cycle. The active site has a unique geometric shape that is complementary to the shape of a substrate molecule, similar to the fit of puzzle pieces.

The substrates bind to a region on the enzyme called the active site. The active site is precisely shaped to hold specific substrates. Beta-galactosidase is one of the three genes in the lac operon. A lac operon is an operon required for the digestion of lactose in bacteria cells. B-galactosidase converts lactose, a disaccharide, into glucose and galactose, monosaccharides.

All enzymes are under the class of protein biomolecule. Amino acids are the basic units that are combined to make up an enzyme. The biomolecule that stores information is a Nucleic Acid. The specific 3-D region within an enzyme is called the active site. The chemical

In this multi-step experiment, there were multiple hypotheses which were stated. The first hypothesis was that if an enzyme catalase is added to Hydrogen Peroxide, then the temperature of the H2O2 will increase. The other two pertained to an enzyme being affected. If boiled catalase is mixed with Hydrogen Peroxide, then there will be a larger temperature jump than if only pure catalase was added. The third hypothesis was if catalase with acid is added to H2O2, then the temperature will have a smaller jump than if only pure catalase was added.

Enzymes speed up chemical reactions enabling more products to be formed within a shorter span of time. Enzymes are fragile and easily disrupted by heat or other mild treatment. Studying the effect of temperature and substrate concentration on enzyme concentration allows better understanding of optimum conditions which enzymes can function. An example of an enzyme catalyzed reaction is enzymatic hydrolysis of an artificial substrate, o-Nitrophenylgalactoside (ONPG) used in place of lactose. Upon hydrolysis by B-galactosidase, a yellow colored compound o-Nitrophenol (ONP) is formed.

Introduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).

Enzymes consist of an active site, this active site is unique to the substrate which it binds to. The active site is a tertiary structure which defines what substrate can bind to the active site. The active site is therefore highly specific. The structure and function of enzymes are compared to the lock and key hypothesis, where the lock is the enzyme and the key being the substrate. Another theory which has been presented is the induced fit hypothesis, where the tertiary structure in the active site changes slightly when bonded to the substrate to strengthen the bond between the active site and the substrate.

An enzyme is a biomolecule that acts as a catalyst in biochemical reactions (1). Enzymes are commonly used in many products and medications. Enzymes function by flexibly binding to active sites in substrates (reactants). This binding is weak non-covalent interactions.

Along with being found in plants, they are also present in liver cells, kidney cells, leukocytes and erythrocytes. For the concentration of enzyme experiment, the hypothesis was if the concentration of an enzyme increases, then the enzyme activity will increase as well. The hypothesis was proven to be true, because there are more enzymes to react with substrates. For the enzyme—factors affecting, the hypothesis concluded was if the temperature increases, than the enzyme activity will increase. This however was proven wrong, because enzymes become unstable at higher temperatures.

The spot where these substrate atoms fit is known as the active site (BBC News. 2014).With the active site; it determines the shape, charge and whether the enzyme is hydrophobic or hydrophilic. On the off chance that the protein's state changes, its active site might not work. This means that the chemical has been denatured (BBC News. 2014).They can be denatured by high temperatures or extremes of pH. When there are changes in the pH or temperature, it can modify the shape of an enzyme which causes the reaction to slow down.

Digestion is a form of catabolism process of breaking down food physically and chemically large food molecules in to smaller components. Chemically digestion is carried out using enzymes and hormones with in different segments of the digestive tract. The presence of enzymes in the digestive tract helps breakdown polymeric biomolecules into individual monomers. This process is crucial for surviving because cells cannot use nutrients the way they were consumed without being metabolized. Nutrients need to be small enough to be absorbed by epithelium of the small intestine and transported by the help of carrier proteins.

An enzyme can be regarded as a catalyst for a biochemical reaction, or more simply, a biological catalyst. Its sole purpose is to increase the rate of a reaction, or speed up the reaction via provision of an alternative reactive pathway, which entails a lower activation energy. Enzymes participate in the reaction itself, enabling the occurrence of an alternate pathways of reaction, but they do not attain permanent changes to their structure or nature, and as a result remain unchanged preceding a reaction. It is then understood, that they can amplify the rate of a reaction, but are unable to effect upon the equilibratory position. Enzymes, unlike chemical catalysts are highly specific in terms of the reactions that they catalyse.