The voltammetric measurement has been performed by transferring the oxidized TMB solution to the 96X carbon electrode. Thus, linear sweep voltammetry were measured by sweeping the potential from +6.00 to -2.00 mV. The calibration curve couldn 't be plotted with the obtained voltammograms, therefore, the cocaine-benzoylecgonine antibody concentration has been raised to 0.05 µg/ml (10 fold more) and the tracer dilution ratio to 1:10000. That leads to increase the absorbance (OD) detected by ELISA to 4. The obtained voltammograms were investigated and the calibration curve was plotted. It showed non linear four parameter logistic relationship fit between the concentration of cocaine and the potential applied. As shown in the figures, with increasing the OD, the potential peak position shift with different cocaine concentration. …show more content…
Consequently, the concentration of cocaine benzoylecgonine antibody and BEC-HRP conjugate concentration was optimized to enhance the sensitivity of the assay. Therefore, cocaine-benzoylecgonine antibody concentrations (0.05, 0.025, 0.0125, 0.005µg/ mL) were combined with different dilution of BEC-HRP conjugate (1:1000, 1:5000, 1:10000, 1:15000). Non-specific binding was produced with BEC-HRP conjugate dilution of 1:1000. 0.0125 µg/ml cocaine-benzoylecgonine antibody and tracer dilution of 1:10000 were the optimized concentration to produce the lower OD of 2.3. As a result, the C value dropped from 1.2 to 0.3 using carbon working electrode plate. Since, the absorbance and the c value had been raised, the sensitivity of the assay compared to ELISA was expected to decrease. However, by measuring the precision profile of 0.0125 µg/ mL Antibody concentration and the conjugate of dilution (1:15,000), the LOD of 14 ng/L using electrochemical detection was obtained compared to 10 ng/L using
For this lab, zeolite and magnetized zeolite were synthesized and compared with charcoal to find out with would be the most effective in the sequestering of Procion Red dye. Finding the concentration and absorbance of each zeolite, magnetized zeolite, and charcoal, along with a calibration curve, the best adsorbent is determined. Charcoal was the overall best sequestration of the Procion Red dye, since the adsorbent was highest compared to the others. Introduction Pollution has increased in the environment over the years, so the purpose of this experiment is to find the best adsorbent of chemicals to reduce the pollution.
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
Post #2: Drug Test Results 1. How did you score on the drug quiz? Better than expected or not as well as expected? The first time I took the drug quiz I made three out of five, which was surprising considering the information on the quiz was very detailed.
The mobile phase used was a mixture of ammonium acetate buffer and acetonitrile at a ratio of 400:600. A flow rate of 1 mL/min was maintained, and the detection wavelength was 292 nm (22). The required studies were carried out to estimate the precision and accuracy of the HPLC method and were found to be within limits [percent coefficient of variation was less than 15%]. Sample preparation briefly involved 0.4 μ membrane filter through which the sample was filtered, diluted with mobile phase, and 10 μL was spiked into
Cocaine changes the assembly of NMDA receptors, meaning the NMDA receptors are altered so much that calcium ions can not move through, the channel may not stay open and the ability of cocaine to produce plasticity. The NMDA receptor is important in understanding some of the mechanisms associated with the effect of drugs on the brain. Synaptic plasticity is the ability of neurons and their connections to change.
Next, a 10 mL beaker is filled with 3 mL of HCl and measure 10 mL of ionized water into a 140 mL beaker. Carefully turn on laboratory burner and start cleaning the Nichrome wire by dipping it into concentrated HCl acid. Hold the Nichrome wire on top of the flame and repeat the step until the wire doesn 't show any color. When the wire is clean, dip the wire again with some of the acid and dip it into the solution with the unknown compound in it. Place the wire back into the flame again and observe the color of the flame.
Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes.
The purpose of this experiment was to learn about metal hydride reduction reactions. Therefore, the sodium borohydride reduction of the ketone, 9-fluorenone was performed to yield the secondary alcohol, 9-fluorenol. Reduction of an organic molecule usually corresponds to decreasing its oxygen content or increasing its hydrogen content. In order to achieve such a chemical change, sodium borohydride (NaBH4) is used as a reducing agent. There are other metal hydrides used in the reduction of carbonyl groups such as lithium aluminum hydride (LiAlH4).
Drug testing has become a mandatory task in nearly every workplace, no employer would like to employ personnel with unacceptable habits of drug addiction. Employee drug testing is not about catching employees using drugs, it’s about preventing the use of drugs at the work place in order to maintain a healthy environment. Drug testing is a moral obligation and responsibility to keep the workplace safe for all the employees, customers and other related personnel. Common reasons employers implement drug testing is to- • Deter employees from abusing alcohol and drugs • Prevent hiring individuals who use illegal drugs • Be able to identify early and appropriately refer employees who have drug and/or alcohol problems • Provide a safe workplace
1.1 Abstract The purpose of quantitative analysis of protein using a spectrophotometer is to measure the concentration of proteins in a given sample. The experiment is conducted by laboratory method (Biuret Test) and using spectrophotometer to analyze the absorbance of reactants at 540 nm, hence determining the concentration of the proteins in a given sample. The purpose of stopped enzyme assay to study B-galactosidase is to determine the effect of temperature and concentrations of substrate on enzyme activity.
Method: The organ bath file was opened and we examined tissue type and Agonist and Antagonists of different drugs which were available. Guinea pig ileum was selected as tissue type and Acetyl choline as agonists. Then different amount of acetylcholine were added to the organ bath which produced different results and finally, a log – dose response curve for acetyl- choline was constructed with the X axis corresponding to drug dose (Drug concentration) and the Y axis corresponding to response (gms).
The Rmaz and EC50 of histamine control were 16.49gms and 2.093 x 10-7M respectively. In figure 1, when 5 x 10-9M, 5 x10-8M and 5 x 10-7M of mepyramine were added, the EC50 of histamine was increased to 6.254 x 10-6M, 4.752 x 10-5M and 0.0002428M respectively. The Rmaz of histamine in the presence of final bath concentration (FBC) of 5 x 10-9M, 5 x10-8M and 5 x 10-7M antagonist either mepyramine or drug A were remained almost constant in Figure 1 and 2. On the other hand, the EC50 of histamine was increased to 3.455 x10-5M, 0.0001693M and 0.0006647M during the presence of FBC of 5 x 10-9M, 5 x10-8M and 5 x 10-7M drug A respectively. Hence, higher histamine’s concentrations were needed to achieve EC50 as the antagonist’s concentration increased.
For this, 25 mL of pH 4 and pH 7 buffers were taken in 50 mL or 100 mL beakers. The buffer solutions were saved in case the electrode needed to be re-calibrated later on. The potassium acid phthalate solution was titrated with 0.1 M NaOH.
Abstract The unknown concentration of benzoic acid used when titrated with standardized 0.1031M NaOH and the solubility was calculated at two different temperatures (20◦C and 30◦C). With the aid of the Van’t Hoff equation, the enthalpy of solution of benzoic acid at those temperatures was determined as 10.82 KJ. This compares well with the value of 10.27KJ found in the literature.
Introduction Drug use in sports has always been a controversial issue. With athletes pushing for the top podium position, performance enhancing drugs can be extremely enticing. One of the main types of drugs used by athletes are stimulants such as cocaine, amphetamines or ecstasy. These can create unfair advantages in sports. To keep sports even and fair, certain drugs became prohibited.