The purpose of transcription is to transcribe the information off of the DNA strand into messenger RNA for translation. There are three stages that take place in transcription; initiation, elongation, and termination. The first stage is initiation, which starts the whole process. During initiation the enzyme RNA polymerase binds to the promoter, which signals the starting point of transcription. When the RNA polymerase binds to the promoter the DNA strands unwind and transition from double-strands into single-strands. In the next step, elongation, the RNA polymerase adds nucleotides to the 3’ end of the growing RNA chain as it moves down the strand. While this is happening the new RNA molecules that have just been made come off of the DNA template and the previous …show more content…
This simultaneous transcribing can help the cell make larger amounts of the encoded proteins by increasing the amount of mRNA that is transcribed. The growing RNA strands are like tails coming off of each RNA polymerase that represent how far the enzyme has traveled from the starting point. The next step is termination, which is basically the ending of transcription. The process of termination varies between bacteria and eukaryotes. In bacteria, transcription will get to a terminator sequence also known as a termination signal. This will signal the RNA polymerase to detach from the single DNA strand. In eukaryotes, the RNA polymerase II reaches a sequence of six nucleotides which is called the polyadenylation signal sequence (AAUAAA) and is bound right away by proteins in the nucleus. Later on down the strand the proteins from the nucleus cut off of the RNA polymerase and releases the pre-mRNA. The cleavage marks the end of the mRNA, but the RNA polymerase continues to transcribe until the enzymes meet up with it and it disconnects from the DNA
Nucleolus- the nucleolus synthesizes ribosomal RNA (rRNA). Afterwards, these are put together with the proteins produced in the cytoplasm to create ribosomal units. 3. Nuclear Envelope-
Anaphase occurs next in which the cell’s centromeres divide and the sister chromatids separate and move to the opposite sides of the cell. Then comes telophase in which the nuclear membrane begins to reform and the chromosome begin to decondense. Also the spindle fibers disappear. We end this
rRNA forms a part of both subunits on a ribosome, in which proteins are assembled. tRNA take amino acids to the ribosome and matches them to the coded mRNA message. 1c. Infer: Why is it important for a single gene to be able to produce hundreds or thousands of
Today, we know that protein synthesis includes two processes, transcription and translation. In transcription, DNA is copied, split, and converted to mRNA. In translation, the mRNA translates the tRNA. The tRNA then goes to get an amino acid. In the book, James Watson described what he thought protein synthesis was
The chromosomes then split into two sister chromatids which the centromeres hold together. This therefore mean that there are two sets of sister chromatids (four chromatids) in the two chromosomes. Two non-sister chromosomes cross over as the other two remain. Secondly, in metaphase I, chromosomes line up at the center of the spindle fibers in pairs then the third phase, Anaphase I begins when equal amounts of chromosomes divide. On the last phase, telophase I, the daughter cells completely divide, chromosomes disappear, and the nucleic membranes forms.
The newly made mRNA strand travels out of the nucleus to a ribosome where the directions can be made into a protein. A ribosome is composed of one large and one small subunit that assemble around the mRNA. The mRNA now passes through the ribosome. Now, amino acid building blocks are carried into the ribosome attached to specific transfer RNA (tRNA) molecules. The small subunit of the ribosome arranges the mRNA so that it can be read it segments of 3 nucleotides.
The sister chromatids are pulled towards oppsite poles of the cell. (http://andrewhulse.weebly.com/archive-blog---life-in-room-213206209/archives/01-2014) Telophase:the chormatids are now called chormosomes. The nuclear envelope reforms arounds the two sets of chromosomes to form two new nuclei and in each nucleus the nucleolus reforms. The spindle fibres disappear and the chromosomes become uncoiled, elongated and are no longer visible.
Through the experiment of building DNA, I knew more about the composition of DNA, and the way and order of deoxyribonucleotides. Through the DNA transcription and translation experiments, I learnt that mRNA is the transmitter of genetic information. I also knew that a genetic code corresponds to an amino acid. This made me have a deeper understanding of the process from genetic information to protein. Overall, I learnt that DNA is insoluble in ethanol; lambda phage DNA was digested by Hind III and EcoR I in the Sample 3E and was digested by Hind III in the Sample 4E. DNA can be visualized by agarose gel electrophoresis, and the direction of DNA synthesis is from carbon 5 to carbon
It is made up of nucleotides ( Adenine,Thymine, Guanine, Cytosine) that are connected together to form the double helix molecule. Transcription and translation happen, respectively, in the nucleus and in the ribosome to produce the protein. Similarly, these two activities make different products however, they all develop from the DNA located in the nucleus. According to the textbook, after the DNA strand breaks apart in our spot, the RNA (Ribonucleic Acid) copies all the genetic information to be delivered outside the nucleus for the rest of the formation of protein. With no doubt, this reports that DNA is at the head of the operation concerning proteins.
(Fig. 7–1c) are components of nucleotides and nucleic
In this prokaryotic specific mechanism, termination occurs by the formation of a stem loop hairpin structure which occurs at a palindrome. Palindrome A palindromic sequence occurs where a series of base pairs in the 5' to 3' direction on the DNA or RNA strand is repeated in the inverse sequence further along the same strand. This is the termination signal for the Rho Independent Termination. These sequences are able to base pair together as the RNA strand folds over on itself to form a stem loop structure as it is being transcribes.
And therefore genes are section of DNA containing information that will code for production of a protein. The sequence of bases in the DNA is referred
As the production of histones peaks, the S phase of interphase begins. During the S phase, duplicate copies of each chromosome are synthesized, this processes can take a while as many eukaryotes have a large number of very long chromosomes. For example, human cells have to duplicate 46 chromosomes each one thousand to five thousand times the diameter of the nucleus in length when stretched. After
DNA and RNA Introduction DNA and RNA are one of the most significant macromolecules in a cell. The transition of information from DNA to RNA and protein determines absolutely all features of a living cell: its size, shape, function and time of death. There are three main sequential mechanisms, by which this transferring of information occurs within a cell: DNA replication, transcription and translation. DNA Replication DNA is a double-stranded macromolecule, which consists of sugar (deoxyribose), phosphate groups, and four types of nucleotides: adenine, thymine, cytosine, and guanine. This molecule also has two different ends: 3’ and 5’; its strands are antiparallel.