Tlc Lab Report

While the solution dissolved, 50 mL of distilled water was added to a 150 mL beaker and heated on the hot plate. When the solution started to boil 2.65 grams of Na2SiO3*5H2O was added to the beaker with a stir bar and heated to a gentle boil. When both solutions began to boil, the sodium silicate solution was slowly added to the sodium aluminate. The solution was kept at 900C for 60 minutes and stirred with stir bar. After 60 minutes, the zeolite solution was cooled for 5 minutes and for the magnetized zeolite , 0.78 grams of FeCl3 and 0.39 grams of FeSO4*7H2O was added to the flask and stirred until the iron parts dissolved.

Next, about 10 mL of both solutions, Red 40 and Blue 1, were added to a small beaker. The concentration of the stock solution were recorded, 52.1 ppm for Red 40 and 16.6 ppm for Blue 1. Then, using the volumetric pipette, 5 mL of each solution was transferred into a 10 mL volumetric flask, labelled either R1 or B1. Deionized water was added into the flask using a pipette until the solution level reached a line which indicated 10 mL. A cap for the flask was inserted and the flask was invented a few times to completely mix the solution. Then, the volumetric pipette was rinsed with fresh deionized water and

Using two test tubes, label one “s” for substrate and the other “e” for enzyme. The substrate tube should contain 7 mL of distilled water, 0.3 mL of hydrogen peroxide, and 0.2 mL guaiacol and the enzyme tube should contain 6 mL of distilled water and 1.5 mL of peroxidase. Combine the materials of the substrate and enzyme tubes, mix the two using a clean transfer pipette, transfer a portion into a cuvette so that the cuvette is about half-full then cover the top of the cuvette with Parafilm and then place it in the spectrophotometer and record absorbance. Remove the cuvette and repeat recording absorbance at 1, 2, 3, and 4 minutes. Be sure to mix the cuvette and clean its surface with Kimwipes before each reading.

While waiting, label the four petri dishes B lactamase - pGlo, B lactamase/ ampicillin -pGlo, B lactamase / ampicillin +pGlo ,and B lactamase/arabinase/ ampicillin +pGlo. After labeling the petri dishes, remove -pGlo and +pglo test tube containers from the ice cup and place them on the heat rack (at 42℃). Only leave the test tubes on for 50 seconds. After

This enables a substance to be confirmed within a solution. With the intention of determining the unknown ion within the solution, the solution was placed inside of a test tube. It was significant to come across the correct order to precipitate the unknown ion within the solution by utilizing the solubility chart to determine a anion that can be used to precipitate the substance. It is important to note that certain solutions such as Na2SO4, Na2CO3, NaOH were used, in that order, to find out which ion is within the original solution. First, a few drops of Na2SO4 were added to the test tube consisting of the unknown substance.

Hence, ferrocene was the yellow layer and the acetyl ferrocene is the orange layer. Column chromatography can be separated from Part A through the same additions. The nonpolar compounds can be separated with addition of hexane. The polar compound such as diphenyl methanol would be added with ferrocene mixture. First, the benzophone would be separated in one flask.

In addition, Elution Volume (Ve) came out to be 5.00 ml. It was calculated by adding all of the fractions from beginning to the last fraction that contained yellow food dye. Yellow food dye was the smallest substance in size which made it easy for it to fit in all the pore sizes

3mL of the liquid in each of the vials were added into cuvettes and measured in the spectrophotometer. Before each time point the photo spectrometer was zeroed using a cuvette with 3mL of distilled water. If any of the results were considered unusual the machine was zeroed again and the sample was retested. The results from the spectrophotometer test were recorded in a table. The experiment was repeated six times to gain a sample size of six.

I. Purpose: To experimentally determine the mass and the mole content of a measured sample. II. Materials: The materials used in this experiment a 50-mL beaker, 12 samples, a balance and paper towels. III.

Glacial acetic acid and acetic anhydride were added to the mixture while refluxing, which converted the lime colored solution into a clear mixture. The flask was cooled in an ice bath and the solution

After (NH4)2CO3 was added to the basic solution, heat is applied to the test tube. It was then centrifuged and the liquid was discarded into a waste beaker. The precipitate was then washed with deionized water and centrifuged once again. Acetic acid was then added to the solid precipitate to dissolve it, resulting in a clear solution. Potassium chromate (K2CrO4) was added in step 12 because the chromate (CrO42-) ion and the unknown cation.

Unlike in TLC the different components can be separated and collected into different fractions. The stationary phase that we will use is aluminia (Al2O3), and as before the mobile phase will be the organic solvent chloroform:methanol (CHCl3/CH3OH). Similar to TLC, the mixture interacts with the solid and liquid phase and is brought down through the column by a flow of the mobile phase. Each component is carried down at a steady rate, which depends on the solubility in the mobile phase and how tightly it binds to the stationary phase, so that as the mixture is transported the components separate. The aluminia is very polar so polar components bind more tightly to it, this means that the higher up the fraction of the component that more polar that component is.

For TLC profiling, 4 TLC plates were prepared for the testing of each solvent. As shown in Figure 1, the green food dye was placed at the bottom center, specifically 0.5 cm away from the bottom of the plate, with the use of a capillary tube. Each one of the silica plates were then vertically placed in a small beaker with its inside surrounded by a filter paper saturated with the solvent to be tested and a small amount of the same solvent at the bottom. The TLC plate was then taken out when the rising solvent was about to reach the top of plate. The ammonia: 1-butanol solvent was tested 7 times due to some personal

First, add three milliliters of water as well as three drops of lugols iodine into a test tube and the pipette 2.5 milliliters to be the blank in this experiment. Have four flasks and number them one through four and add 22 milliliters of water and two milliliters of starch solution. Acquire four test tubes and pipette one milliliter into each test tube and then put them on ice to chill. Add three drops of lugols iodine to eight test tubes. place flask numbered one as well as the test tube numbered one in an ice bath for five minutes.

The solution was warmed to 65oC with constant stirring on the magnetic stirrer till the solid was completely dissolved. Stirring was stopped and 0.4g of charcoal was then added to the solution. After which the solution was transferred to a Buchner funnel with a moist filter paper and the clear filtrate was collected via suction filtration. The residue was washed with warm solution of 10.035g of KI and 15ml of deionized water. 200ml of water was then added to the filtrate in a 500ml beaker with constant stirring.