Thin Layer Chromatography Lab Report Miranda Tyree Lab Partner: Caitlin Price TA: Yi Zhou Lab Time: Thursday, 12 pm Lab Room: 207 Date: 09/23/2016 Abstract Experiments were performed to determine the active chemical ingredients in Tylenol and Anacin. The active chemical ingredients were determined by performing thin layer chromatography of four substances: Tylenol, Anacin, acetaminophen, and acetylsalicylic acid. The identity of the ingredients was then confirmed by using the co-spotting thin layer chromatography technique. Tylenol had Rf values of 0.566 and 0.184, Anacin had Rf values of 0.19 and 0.72, acetaminophen had an Rf value of 0.566, and acetylsalicylic acid has an Rf value of 0.79. The active chemical ingredient in Anacin was …show more content…
Once with Tylenol, Anacin, acetaminophen and acetylsalicylic acid samples spotted separately and once with the unknown samples co-spotted with the known samples. The goal is to identify the main chemical components in Anacin and Tylenol, either acetaminophen or acetylsalicylic acid. Silica (SiO2) gel will be used as the stationary phase and an ethyl acetate (C4H8O2) containing 0.5% acetic acid (CH3COOH) solution will be used as the mobile phase. The molecular structures of the stationary and mobile phases are shown in Figures 1 and 2. The stationary phase is polar protic and the strongest intermolecular force present is hydrogen bonding. The mobile phase is aprotic, with the strongest intermolecular force being dipole-dipole interaction. While both acetylsalicylic acid and acetaminophen are polar substances and should interact strongly with the polar protic silica, acetaminophen is the more polar of the two (more hydrogen bonding) and as such should interact more strongly with the silica gel than the acetylsalicylic acid. This will result in the faster adsorption and slower desorption of acetaminophen compared to acetylsalicylic acid. Thus the acetaminophen distance traveled (and as a result the calculated Rf value) will be lower than that of acetylsalicylic acid . The molecular structures of acetaminophen and acetylsalicylic acid are shown in Figures 3 and 4. Ultimately, comparing the Rf values of Tylenol …show more content…
This was due to the stronger intermolecular forces between acetaminophen (hydrogen bonding from both the amide and phenol) and the solid stationary phase compared to the weaker intermolecular forces between acetylsalicylic acid (hydrogen bonding from the carboxylic acid) and the solid stationary phase. This resulted in faster desorption and slower resorption of acetaminophen compared to acetylsalicylic acid. Overall, thin layer chromatography is a useful tool for determining unknown chemical components in a substance. Conclusion The purpose of this experiment was to determine the active chemical component in Tylenol and Anacin using thin layer chromatography. The active chemical ingredient in Tylenol was determined to be acetaminophen while the active chemical ingredient in Anacin was determined to be acetylsalicylic acid. Works Cited Gupta, H. (2016). [Silica Gel Structure]. Retrieved September 23, 2016, from http://www.biologydiscussion.com/micro-biology/chromatography/thin-layer-chromatography-with-diagram/18037 Thin Layer Chromatography Lab Package [PDF]. (2016). Tempe: Arizona State
To begin, the solubility of the unknown compound in water was tested. If the compound is soluble in water, it can be inferred that it is either a polar covalent or ionic compound.
Imagine going days without feeling safe to take any medicine. A popular event that happened in 1982 was the Chicago Tylenol Murders. A group of victims were killed by taking the everyday medicine that has been poisoned. The Chicago Tylenol Murders of 1982 are very dangerous because of the sudden first killings, main suspects, and testing the Tylenol. Tampered Tylenol was found at a couple different stores and there was a small chance for a victim to get a poisoned tablet.
Case Study 1 Hepatic Failure Dalia O. Smith Allan Hancock College Abstract Cirrhosis of the liver is a chronic degenerative disease characterized by liver fibrosis and decreased hepatic function. Fibrotic tissue, seen in cirrhosis patients, impairs the function of the liver which eventually leads to liver failure. Although cirrhosis is often associated with prolonged alcohol use, exposure to toxic substances can also cause cirrhosis of the liver. Amongst those toxic substances is acetaminophen, and levofloxacin, both commonly used drugs.
Lab Report 5: Acetylsalicylic Acid (Aspirin) Synthesis Name: Divya Mehta Student #: 139006548 Date Conducted: November 19th 2014 Date Submitted: November 26th 2014 Partner’s Name: Kirsten Matthews Lab Section: Wednesday 2:30 L9 IAs Name: Brittany Doerr Procedure: For the procedure, see lab manual (CH110 Lab Manual, Fall 2014) pages 96-98. Wilfrid Laurier University Chemistry Department. Fall 2014. Acetylsalicylic Acid (Aspirin) Synthesis.
ABSTRACT: Pantoprazole sodium is an antiulcer drug. This compound inhibits gastric acid formation and thereby it is very efficient for the treatment of gastric and duodenum ulcers. But pantoprazole sodium is acid labile drug that will degrade in acidic environment of stomach resulting in therapeutic inefficacy. Hence it is necessary to bypass the acidic pH of the stomach which can be achieved by formulating delayed release dosage forms (single unit or multiple units) by using different enteric polymers The present study was an attempt to formulate and evaluate enteric coated tablets for pantoprazole sodium sesquihydrate.
The results obtained for the volume neutralized by each antacid were 45.28 mL (Alcalak), 11.70 mL (Alka-Seltzer)
Cotton wool was placed into the funnel; this was followed by pouring hot water onto the cotton wool before filtering the dissolved paracetamol crude solution to remove any insoluble materials. Watch glass was placed on beaker containing filtrated solution and the solution was allowed to cool slowly. After the filtrated solution had cooled up, it was placed into a beaker containing cold water. The corners of the beaker containing filtrated were scratched to allow the crystals to form. The purified paracetamol was collected by filtering through a funnel containing cotton wool.
Amino acids are known as the building blocks of all proteins that consists of 20 amino acids which are found in within proteins convey a vast array of chemical versatility. Amino acids are comprised of a carboxyl group and an amino group that attached to the same carbon atom which is the α carbon. They vary in size, structure, electric charge and solubility in water because of the variation in their side chains (R groups). Detection, quantification and identification of amino acids in any sample constitute important steps in the study of proteins. An amino acid that bonded directly to the alpha-carbon have an amino group is called alpha amino acids.
The mean percentage recovery was found in the range of 101.1%-101.84% and 101.84-102.84% for the Absorbance correction method and 99.93-101.14 and 101.62-102.57 for the absorbance ratio method, for ibuprofen and Famotidine respectively at three different levels of standard additions. The precision (intra-day, inter-day) of methods were found within limits (RSD s law in the concentration range of 20-140μg/mL and 2-10μg/mL for ibuprofen and famotidine
This technique is used to isolate prooteins, enzymes as well as antibodies from m mixtures. Partition Chromatography: Here the stationary phase is a thin liquid film either adsorbed or
TYLENOL CRISIS JOHNSON & JOHNSON Johnson & Johnson is an American multinational medical devices, pharmaceutical and consumer packaged goods manufacturer founded by two brothers James and Edward Mead Johnson in 1886. Later Robert Wood Johnson too joined them with the effective leadership that the company required. Antiseptic Surgical dressings were among their first products. Tylenol is an American brand of drugs advertised for reducing pain, reducing fever, and relieving the symptoms of allergies, cold, cough, and influenza. The active ingredient of its original flagship product is acetaminophen, an analgesic and antipyretic, it is commonly known elsewhere in the world by its international nonproprietary name, paracetamol.
Column chromatography set-up After setting up the column, 2 10-ml of the chosen solvent was obtained and was placed in two separate test tubes. Using a dropper, ~0.5 mL of the food dye was put into the column by dropping it at the side of the column in a circular motion. The chosen solvent was then added just after the green food
The prices for a bottle may vary due to various reasons such as strength, brand names, or even the consistency, such as tablets or gummies. The problem here is that there are so many types of antacids at a variety of prices however, how effective the tablets are tends to be unknown. Due to this, the purpose of this lab is to assist consumers in deciding which type of antacids to buy based on the cost and how effective the tablets are at neutralizing the acid found in the stomach. This will be found by performing two different titrations where the antacid will first be reacted with hydrochloric acid and then this will be reacted with sodium hydroxide. It is hypothesized that the Life Brand bottle of antacids will be the most cost-effective because it is the cheapest bottle and it claims to have the same amount of tablets as the Tums Regular Strength with the same amount of calcium carbonate per tablet as the
The purpose of the study is to determine the association of acetaminophen and development of asthma five to seven years later in children who used acetaminophen for fever at least once a month in the first of their life. Independent variable: use of acetaminophen in children for their first year of their life Dependent variable: risk of developing asthma five to seven years later. Hypotheses: 1) There is a relationship between the use of acetaminophen and the risk of developing asthma in children 2) There is no relationship between the use of acetaminophen and the risk of developing asthma in children. Definition of terms: Acetaminophen (Tylenol) – belongs to a class of drugs called analgesics and anti-pyretic.
Ibuprofen is known as (2RS)-1[4-(2-methylpropyl) phenyl] propanoic acid. It is a non-selective inhibitor of cyclo-oxygenase-1 (COX-1) and Cyclooxygenase-2 (COX-2). 1 It may has weaker anti-inflammatory properties than some of other NSAIDs. However, it has a noticeable analgesic and antipyretic function. The inhibitory actions on cyclo-oxygenases are involved in the synthesis of prostaglandins.