Prodigioin Synthesis Lab Report

Differential media allows for the differentiation between two similar micro-organisms through how the bacteria may handle certain compounds found in the media or the different reactions that may take place when the bacteria is exposed to the medium (3). Selective media on the other hand allow only certain microbes to grow. This is due to the plate containing a limited amount of nutrients, compounds and chemicals that will deter the growth of certain bacteria (3). Dyes, antimicrobial substances, salts, certain growth inhibitors and, antibiotics are also found on this type of medium (3). The differential and selective media mentioned in this lab are as follows:

I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided

There has recently been an outbreak of a new disease in South America known as Ashella schmiddy. Recent studies that have been released have shown that A. schmiddy is a highly infectious flesh eating bacterium. A. schmiddy is a Gram Negative(-) bacillus, and it’s preferred portal of entry is the skin. It has been proven that once the bacteria is introduced to even the smallest cut or abrasion on the skin, infection quickly follows. One thing that makes this new disease so worry-some is the number of invading microbes that it takes to infect 50% of the population, which is only 15-45 cells, which is an extremely low dosage.

This leads me to believe that the colonists packed up everything they had and left for croatoan. The mark on the tree is also very

Crystal violet was then added for 60 seconds before being washed off with water. The mordant, Gram’s Iodine, was added for another 60 seconds before getting washed off with water. The heat fixed smear was then washed with 95% alcohol until the wash ran clear, leading to the final step of adding Safranin for 45 seconds before being rinsed with water. The slide was finally blot dyed with bibulous paper before it was placed under a microscope to observe the color and shape of the bacterium. 2.2 Litmus Milk Reaction

The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not

The putrid smell of Escherichia coli is one that is immediately identifiable to the few lucky individuals who recognize its scent. It is also an aroma with which I became intimately sensitive to as I shuttled petri dishes of the bacterium in and out of an incubator. While my classmates shied away from the task of handling the pungent bacteria used in our recombinant DNA experiments, I took to the task eagerly, anything that would take me one step closer to my goal of researching. I had the opportunity to learn about lab techniques and cutting edge biology concepts the summer before my junior year, in an extracurricular biotechnology class at Northwestern University’s Center for Talent Development. The class, a three week crash course in the

Introduction: This experiment includes the use of a genome editing system in order to make E.coli cells resistant to the antibiotic streptomycin. The system uses a protein called Cas9. Guide RNA is used to guide the Cas9 to the targeted sequence of DNA. The Cas9 finds the match to the gRNA in the cell’s DNA and cuts it out. As the cell tries to repair the DNA, it uses the template DNA that was inserted into the cell, and results in the modified DNA.

Escherichia Coli 0157: H7 This paper will specialize on a specific type of bacterial foodborne illness caused by the bacteria Escherichia Coli. E. coli was discovered by Theodore von Escherich in 1885. E.coli is a natural found bacteria that lies throughout the intestinal tract of warm blooded animals and comes in many forms only one of which is deadly. This form is E. coli 0157:H7 which can be caused by direct exposure to fecal matter to kill this rouge

Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.

The sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used. The plate was incubate with lid up on the bookshelf at room temperature for 48 hours.

Nguyen Nguyen Professor Microbiology 1 May 18th, 2016 01MW – Staphylococcus Epidermidis The Staphylococcus Epidermidis is classified as bacteria. Scientists reckon it to Firmicutes phylum and adjust it in Bacillales order of Bacilli class. This bacteria belongs to Staphylococcaceae family.

Spectral analysis of M. purpureus red pigment Spectral analysis of pigments produced by M. purpureus on corn starch medium shows absorbance peaks at 420–430 and the highest peak was at 500nm (Fig. 2). In agreement with our results; Silveira et al (2011) found that spectral analysis of pigments

it is use in feed supplement farming that helps to promote animal growth as an alternative antibiotics. Bacillus subtilis can produce a variety of antimicrobial to compete other microorganisms by either killing them or reducing their growth rate. And subtilis also produce JAA that can be used against various crop diseases which can decrease significantly the growth and productivity of the crops (Wertheim et., al,2o14). Pseudomonas aeruginosa normally inhabits the soil and surfaces aqueous environment. This pathogen can tolerate a low oxygen conditions and it can survive in low levels of nutrients and temperatures.

The media used in this experiment was Trypticase nitrate broth. The reagents used (A and B) were sulfanilic acid and alpha-naphthylamine (respectively). Using aseptic technique, the bacterium (16A and 16B) were inoculated into labeled broth test tubes. The tubes were incubated for 48 hours at 37 degrees Celsius. When the incubation was complete 5 drops of reagent A and 5 drops of reagent B were added to each of the broths.