In table 1. the pH of 7 has the highest rate of O2 production being 4.41mL/min while the pH with the smallest rate of O2 production being 0.21mL/min is 4. In either direction from the pH7 the average rate is decreasing similar of that to table 2s trend. In table 2. there is a pattern from the temperature of 0.C to 100.C the average change is 2.69mL/min at 0.C then at 23.C it is at its peak being 5.5mL/min but it then decreases again at 4.4mL/min at 37.C. the trend is that in either direction of 23.C the average rate of O2 produced is decreasing. As per table 3. there is a pattern with the enzyme concentration going from 100% to 0%, at 100% the rate of O2 production is at its highest being 7.79mL/min while at 0% the rate of O2 production is at its lowest being 0mL/min. In Table 4. the substrate concentration has the same pattern as the enzyme concentration, at the highest concentration being 3% the rate of O2 production is 7.58mL/min and at the lowest …show more content…
is the results of the effect of enzyme concentration on the potato enzyme. The 100% enzyme concentration had the highest average rate of O2 production being 7.79ml/min. This is because when there is a 100% concentration there is more molecules of the catalase for the hydrogen peroxide to react with. As the concentration is being diluted, the concentration of the catalase is decreasing so the molecules are less likely to collide and react. When the enzyme is at a 0% concentration there should not be any 02 produced and this is where some errors may have occurred. At 0% enzyme concentration there was a 0.58mL/min average rate of O2 produced, there should have been a 0mL/min average rate because if there is no enzyme for the substrate to bump into then there is no reaction to occur. Some errors that could have been the cause of this where not rinsing out the test tubes properly leaving residue of the enzyme left inside or air pockets trapped in the tube may have caused the reaction or appearance of a
7. Will the temperature effect the pH Scale? Hypothesis: The hypothesis of this experiment is that the rate of reaction will increases well as the hydrogen peroxide concentration. If the temperature, pH and enzyme concentration is kept constant then the rate of reaction will start to decrease as well as the hydrogen peroxide concentration. Aim: To investigate the effects of changing the concentration of the enzyme catalase that it has on the rate of breaking down the Hydrogen Peroxide solution.
It was hypothesized that the optimal pH for the enzyme was pH 7 while the 1.0 ml peroxidase would have the best reaction rate. At the end of the experiment the results prove the hypothesis to be incorrect. INTRODUCTION Enzymes are proteins that allow a reaction to speed up. These proteins are made up of monomers known as amino acids.
There are few vegetables and fruits that turns to the color brown if their surface is exposed to oxygen. Once the veggies or fruits been exposed to oxygen, then the browning begins to appear, and electrons and hydrogen will be removed. This happens because of an enzyme called catechol oxidase. The enzyme will act on its substrate catechol to form a yellow compound which then reacts with the oxygen in the air and change into benzoquinone. The more concentration of the enzyme, the more browning appears.
Lab Report -- Relationship on Enzyme activity and substrate concentration Research Question: Is the more concentrated the substrate of hydrogen peroxide is, the shorter the time taken for the paper disc to rise from the bottom of the beaker? Aim: The opposite of hull hypothesis Background Information: This experiment aimed to investigate on the relationship of the substrate concentration and enzyme activity. Enzymes are proteins produced by a cell that acts as catalysts to increase the rate of a specific chemical reaction without changing the reaction itself.
The 0.1% is the concentration amount. Just like temperature and pH, substrate concentration can speed the reaction only up to a certain limit. When we mixed pH 3 enzyme tube with substrate tube, we used 0.3 mL of hydrogen peroxide, but if we were to increase the amount, then the experiment would have been faster. Our
3. Look at your graph for Part B, how does temperature affect enzyme activity? The colder the temperature the greater the reaction. 4. Look at your graph for Part C, how does pH affect the enzyme activity?
Oxygen was being consumed and carbon dioxide was being released because the water level in test tube “G” continued to
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
By using a spectrophotometer to measure absorbance at 420 nm, the rate of enzyme activity after all reactions have come to a stop can be
Introduction In class, a series of experiments were performed that pertained to the enzyme known as catalase, which converts hydrogen peroxide into oxygen. Due to peroxide being toxic to the tissues of both plants and animals, both possess the enzyme catalase, which breaks into two non-toxic compounds: water and oxygen gas. Enzymes are proteins that react to certain substrates to create a product, and continue doing so afterwards. Methods and Materials To test reactions between catalase and hydrogen peroxide, groups of three to four people were formed.
Controlled Concentration of amylase Amount of amylase/starch Ph of the amylase/starch The concentration of the Amylase was kept at 1% at at times throughout the experiment. 5cm3 of both will be used in each reaction. pH of the Amylase/starch will be kept the same.
H20 + 2 O2 This experiment will use 1% catalase solution and 3% hydrogen peroxide solution, both diluted into water so the reaction slows down. Temperature will be controlled in this experiment to change the reaction speed of the enzyme and the substrate, this is what the experiment is looking at. The effect of the temperature will be determined by how much gas is released in two minutes, which will change the pressure inside the test tube and will be measured by a gas
By observing figure 3, the more enzyme that is available, the faster the reaction rate is. The optimal enzyme concentration was chosen based on the R2 values from figure 2. The highest observable rate also had the best R2 number, which was closest to one. This enzyme concentration was used in part 2.
ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.