Chapter-1
INTRODUCTION
1. Introduction
Phosphorus (P) is one of the most essential component of the nucleic acid structure which regulates protein synthesis and plays an important role in biological growth and development. Being the most limiting macronutrient after nitrogen, P plays a significant role in increasing root ramification and strength as well as provides vitality and disease resistance. Along with these essential functions, P is also associated with complex signal transduction, macromolecular biosynthesis, energy transformations and respiration in the plant (Khan MS et al. 2010). Soil P exists in various chemical forms including inorganic P (Pi) and organic P (Po) which differs in their behavior and fate
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Soil microorganisms are effective in releasing P from organic and inorganic pools of total soil P through mineralization and solubilization. The major mechanism of phosphate solubilization is the production of organic acids. The major mechanism of phosphate solubilization is the production of organic acids. Among the organic acids produced, gluconic, formic acid, 2-ketogluconic, citric, oxalic, lactic, isovaleric, succinic, glycolic and acetic acids produced from P- solubilizing bacteria. Production of organic acids results in the lowering of pH in the surroundings and many reports suggests a positive correlation between lowering of pH and mineral phosphate solubilization. Some of the alternate mechanisms suggested are production of chelating compounds, inorganic acids like sulphuric, nitric and carbonic acids. Another attractive application of P-dissolving enzymes is the mineralization of soil organic P compounds through phytate degradation mediated by the enzyme phytase, which specifically causes release of P from phytic acid. Phytate is a major component of organic P in soil. There are several reports of phytase producing organism. Richardson AE (1997) reported insoluble P solubolization by secreting phytase enzyme in Pseudomonas sp. (Richardson AE and Hadobas PA, 1997). Several species of microorganisms are able to secrete …show more content…
Thus, the amount of P liberated by them is generally not sufficient for a substantial increase of in in situ plant growth. Therefore, inoculation of plant by desire microorganism at adequate amount than the normal found in soil is necessary to take advantage of the property of phosphate solubilization for plant growth and yield enhancement (Rodrigues H and Fraga R, 1999).
OBJECTIVES
In the context of above observations, the present investigation was carried out with the following objectives:
1.1. Objectives of the Study
• To isolate the phosphate-solubilize bacteria from rhizosphere of maize plants,
• To detect the phosphate-solubilizing ability,
• To characterize the microorganisms at the phenotypic and genotypic level,
• To study the diversity of phosphate solubilizing bacteria,
• To examine the production of organic acids,
• In addition, this study was to examine the effect of phosphate-solubilizing bacteria as inoculants on plant growth and yield and may be used as biofertilizer.
• We also hypothesize that the combined effect of microbial strain can also give better result as compared to the single
The purpose of this experiment was to analyze the % phosphorous in Miracle Gro. Gravimetric Analysis was used to determine the content of phosphorous in this water soluble fertilizer. The overall goal of Gravimetric Analysis is to form the precipitate MgNH4PO46H2O and isolate it from the aqueous solution by vacuum filtration. In order to obtain the precipitate, a series of dissolution reactions and vacuum filtration were performed. First, Miracle Gro was dissolved with DI water and reacted with activated copper to remove coppers ions in the fertilizer.
Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
In this experiment, we cultivated an unknown specimen containing two microorganisms. The purpose of this experiment was to use a variety of biochemical test previously learned in the lab to identify the unknown bacteria. The identification of unknown bacteria is a major part of microbiology. Microbiologist observe samples such as blood and sputum in the laboratory for the presence of microorganisms. Identifying unknown bacteria is extremely important in clinical settings because it helps physicians find treatment for infections.
If the experiment was completed again, more higher concentrations could be applied to the seeds to see if the data is further supported. Either way the more trials the better and more accurate results, this applies to any experiment. Practical applications of the principles of this investigation may be using the correct concentration for a specific plant or seed. This lab shows that it is necessary to follow the directions and specifications when using the fertilizer miracle
In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
On page 117 paragraph 4 it says “ Phosphorous is found in fertilizer. In 1986, when detriment levels of phosphorous were discovered, water management becomes primary.” Since the sugarcane crop is grown so close to the Everglades the phosphorous found in fertilizer is getting into the
To prepare the solutions a 70% ethanol solution was used to make 40%. This was calculated using the C1V1=C2V2 formula. A photo spectrometer was used to measure, in arbitrary units, the change in membrane permeability of the B. Vulgaris cells. To begin, the B. Vulgaris samples were put into vials containing the distilled water, 40% and 70% Ethanol solutions. As soon as the B. Vulgaris samples were added to the vials a time zero sample was taken from the vials.
1. I would say that a limiting nutrient is anything that is necessary for growth, but which can cause problems if there is an excess. For example, phosphorus is necessary for plants to grow, but too much can probably lead to problems. It is normal for a lake like Lake Erie to have phosphorus in the water, but since there is too much, the algae is growing out of control. Phosphorus is a “limiting nutrient” because it is only required in small amounts for proper growth and balance in the ecosystem.
The plants with 200 ml topsoil were projected to have the highest average height according to the original hypothesis of increased plant growth due to an increase on the amount of topsoil added. Moreover, the original hypothesis was refuted as the plants with no topsoil in the control had a higher average height than the plants with 25 ml topsoil. The mixed red and green color on the leaves of the plants with 25 ml topsoil indicated possible deficiencies in phosphorus which might have slowed their growth. Moreover, the mixed red and green color on the leaves of the plants in both 100 ml and 200 ml topsoil also indicated a possible phosphorus deficiency which may have resulted in the slower growth of the plants despite having greater amounts of topsoil. The experimental results might have skewed due to the over fertilization of the radish seeds which might have led to excess nutrient levels which are unsuitable for radish plants as they require little to no fertilization for growth (Chase, 2018).
Without crops, we have no way to feed the ever-increasing population. While there is not necessarily a cause for alarm in this particular element right now, a report provided by the International Fertilizer Development Center states: “There is no substitute for phosphorus in agriculture, or indeed in life. Phosphate rock is a finite resource—at some point in time the earth’s supply may be exhausted.” (“IFDC Report Indicates Adequate Phosphorus Resources Available to Meet Global Food Demands.” IFDC.
Introduction: In this task I will be researching the effect that acid rain has on the rate of plant growth. Acid rain is any type of precipitation with a high pH, with high levels of nitric acids. The reason why I had chosen this topic was because acid rain seems to have a great effect on the effect of plant growth, and plants play a very important role in our ecosystem. Acid rain is a major problem in our environment when we are not able to neutralize the acidity.
Bacteria that causes disease are called pathogens. The disease is caused by a poison called exotoxin and endotoxin produced by the bacteria. Another microbial life are protist. They are unicellular eukaryotes. Types of protist includes protozoans and slime molds.
LITERATURE REVIEW Inorganic fertilizers provide rapid nutrition and give plants with the nutrients needed to grow healthy and strong. Inorganic fertilizer, also called as synthetic fertilizer, is manufactured artificially and contains minerals or synthetic chemicals and it is which help a plant to grow faster. Effective use of Inorganic Fertilizers Effectiveness in using these chemicals has good and bad effects on the environment especially on the health of a human being. Fertilizer has turn into essential to modern agriculture to feed the growing population.
Sedimentation and pollution in the streams or rivers causes effecting the life duration and quality of fish and other species. Land degradation are also reducing the water holding capacity of soil on the contrary, increased by flooding and leaching of water and nutrient losses mainly as nitrate form. The other substantial change in land usage is the maintaining of sustainability. According to FAO statistics, 28% of the total earth surface have any limits on its use for agriculture while 28% is too dry, 23% has chemical imbalances, 10% is too wet, 6% is permanently frozen, and remaining 22% the soil is to shallow for use as arable land. Altgouhg there are some discussions about the renewable subject of soil today, generally if the soil formation rate exceed the degredation rate, soil is accepted as renewable, on the contrary the degredation surpasses the formation, soil is accepted as