Pglo Lab Report

The Mut section is expected to have no growth as mutants require the amino acids leucine and valine to grow which is not provided in the minimal medium. Results Figure 2. Testing of mutant mixed with DNA, mutant bacteria and DNA on LB medium Growth was observed on the Transformed (Trsf) section and the Mutant (Mut) section but not on the DNA section. Due to human errors, the photo of our experiment was lost, but we have obtained similar results as from group1.1 and their photo is presented.

To limit the amount of errors or contamination in any procedure lab safety rules, gloves, and the aseptic technique was strictly enforced throughout the experiment. The first step to identifying the unknown bacterium was the Streak Plate Method. This method is used to isolate a pure culture from a mixed culture. Also, this method included streaking a tryptic soy agar (TSA) plate into four quadrants, and later incubating the plate for 24 hours.

In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.

The putrid smell of Escherichia coli is one that is immediately identifiable to the few lucky individuals who recognize its scent. It is also an aroma with which I became intimately sensitive to as I shuttled petri dishes of the bacterium in and out of an incubator. While my classmates shied away from the task of handling the pungent bacteria used in our recombinant DNA experiments, I took to the task eagerly, anything that would take me one step closer to my goal of researching. I had the opportunity to learn about lab techniques and cutting edge biology concepts the summer before my junior year, in an extracurricular biotechnology class at Northwestern University’s Center for Talent Development. The class, a three week crash course in the

• Write down the highlighted numbers. Do you observe a pattern? • Does the pattern grow? What is the reason for this? • Write down the last number (say 53).

chinesis. A construct of R751::Tn4351 (the physical map of R751::Tn4351 and restriction sites are shown in fig. 7) was selected for introduction into F. chinesis to discover if the introduction and insertion of the vector R751 and the transposition of T4351 into the F. chinesis chromosome by a triparental mating occurred. One parent was E. coli GJ342 which carried a helper plasmid, the second parent was E. coli HB101 which contained R751::Tn4351 and the third parent was the F. chinesis target strain. 189 colonies were isolated on LB agar plates which in passage in fresh media were able to grow in 200µgml-1 erythromycin.

Once the time elapsed the tube was viewed and it changed from its green color to a dark blue color indicating a positive test. Based on these results the next test administrated was the motility test. A inculcating needle containing P. aeruginosa was placed into the motility medium using aseptic technique. The tube was then placed into an incubator at 37 degrees Celsius for 24 hours. After 24 hours passed the test was examined and growth was seen radiating from the stab line which indicated a positive test.

and we were excepting to find glucose and salt outside but not starch as the molecule is too big. Generally, the results proved to be reliable in some aspect, i.e. the expected results of glucose and salt found outside the tubing in the surrounding water, but none of the tubes

Task 6 Produce an illustrated article for a magazine that examines E. coli structure, growth conditions and curves and how it colonises a new environment. (M2) Structure E. coli consists of an outer membrane which contains lipopolysaccharides, a periplasmic space with a peptidoglycan layer, and an inner cytoplasmic membrane. Some strains of bacteria are pileated and capable of accepting and transferring plasmid to and from other bacteria and such property enables the E. coli under stress conditions to survive. It can perform complicated metabolism to maintain its cell growth and cell division, even though it has extremely simple cell structure which has only one chromosomal DNA and a plasmid.

Introduction: Genetically modified organisms can be defined as organisms in which the DNA has been changed in a way that does not occur naturally by any reproduction procedure. The enviropig is just one of many organisms that they did experiments on to modify it to have specific (needed) outcomes. The reason for genetic modification is to be able to change a product or organism so that it deliver desirable traits. The enviropig was created to solve the problem of pigs not being able to absorb enough phosphorous from their diet, which then in its turn contributes to the larger factor of pollution.

The needle was inserted in the test tube, making sure not to touch the side of the test tube. Inoculate the media by going through the agar, as the needle is pulled out; drag the needle across the top of the agar. This technique was applied to both the TSI and Citrate

After 48 hours, I observed different growth patterns around the disks. I measured the zone of inhibition of each antibiotic and document them on Microbiology task 3

Keshk (1999) reported that different substrates used can produce different yield of bacterial

However using bacteriostatic agent can have a reverse effect on bacterial selection of which attributes to damage of bacteria in stress condition. To avoid this effect in FDA BAM, the agent added after 4hrs of incubation to allow the injured cells to recover and grow in media, but in ISO 11290 at first step of enrichment, the half concentration of agent is added. In both media the buffering capacity of media is very high and leads to enhanced cell growth and

Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.