Gel Electrophoresis Lab Report

The STR length contrast is what is used to differentiate individuals. Gel electrophoresis then uses the STRs to create a DNA profile. The gel electrophoresis separates the STRs depending on their length and the pattern is then shown in fluorescent gel creating the profile. These profiles are then used by scientist to compare patterns between evidence and or suspects to determine a match or not a match.

The morning of February 24, 2005, the grandmother of 9yr. old Jessica Lunsford called the police in Citrus County, Fla., to report her granddaughter had disappeared from her room the night before. She had tucked her into bed. The only thing that was missing was a stuffed animal that her father won for her at the county fair. Immediately police began to search for Jessica.

In this case they left other objects pthat could have been tested untested. Their could have been DNA, like hair or saliva, left on those objectives. The detectives didn’t know if the objects were 100% not going to have any DNA on it. There was no way for them to find, or understand this, unless they tested these objects. Nevertheless, this isn’t the only time that they had pushed away any possible evidence for DNA testing.

Notably, DNA testing was still in its infancy in the 1990s thereby making it hard for the officers to capture the killer (Hickey, 2016). Additionally, the police did not have the physical evidence that would tie Ridgway as the criminal thereby making it impossible to call for his arrest. The technological limitations brought the case to a standstill but not for long. In 2001, technological advancement brought the case back to light after forensic experts decided to re-examine the DNA evidence compiled for years. The last-ditch effort deployed two means of analysis, the short tandem repeat (S.T.R.) and polymerase chain reaction (P.C.R.) tests making it indispensable to copy the sequence of DNA fragments from the crime scenes (Hickey, 2016).

The apparatus was then closed and turned on to run at 100 volts. Electrophoresis ran for 30 minutes; separating the DNA according to size in the gel. Then the tray with the gel was removed and a stain sheet was placed on the gel for 15 minutes. After 15 minutes that gel was rinsed with water and then set in water. Later, 20 to 30 minutes, the gel was view over a light box to view how far the bands traveled.

Statement of the Problem DNA has become a vital part of criminal investigations. DNA can include and exclude suspects of criminal investigations. During a criminal investigation, all DNA should be collected, properly preserved and tested, but at times this does not occur or the technology was not available for this process to occur. In addition, DNA has become an imperative portion of exoneration cases.

In King, Justice Kennedy referred to the invention of DNA technology as “one of the most significant scientific advancements of our era.” This statement has been criticized, but the impact of DNA technology has been significant. Currently, forensic analysts can use “junk” DNA to identify a person with near certainty. Law enforcement can collect a person’s DNA through saliva. The sample is then uploaded to CODIS, a national network of DNA databases.

1 “substrate” and another “ enzyme.” Instead of using the distilled water, this time you are going to use different pH buffer in the enzyme test tube. In the substrate tube, add 7 mL of distilled water, 0.3 mL of hydrogen peroxide, and 0.2 mL of guaiacol for a total volume of 7.5 mL. For the enzyme tube, instead of distilled water add the pH solution (3) and 1.5 mL of peroxidase which equals a total volume of 7.5 mL. Use the dH2O syringe for our pH solution. To clean the syringe, flush it by drawing 6 mL of distilled water.

One of the pieces of evidence at the crime scene was hair that was caught around a hair tie. The hair tie was found with hair caught in it and lying next to Anna Garcia’s body. A microscope was used to compare the hair that was found at the scene to Anna’s hair and other suspects. After looking at and comparing each suspect’s hair, I was easily able to conclude that the hair belonged to Anna Garcia. The hair that was found at the crime scene perfectly matched Anna Garcia’s

This DNA sample was tested to determine if the DNA left on Mary Sullivan was a match. This proved to not be a match. This shows some suspicion to the final verdict of the

This evidence helped them investigate the case to see if it was the same person who has committed this murder and to capture the person and take them into custody. The critical evidence that they used was the blood that they got from the victims' bodies to figure out if it matches the person who has does this murder. It was the first time that they were using DNA to convict the murderer. The blood semen that they got from each of the victims they used that to punish the murder who done these murders.science and the Forensic Science:After a month there was another occurrence were Stephanie had vanished, another lady disappeared. A seeker found a gravely decayed body of a white female, the remaining parts were scattered by creatures, yet the agents recuperated her skull, middle, and some dress.

This can be done by DNA fingerprinting or by collection of body fluids, such as saliva, semen, urine, blood, skin and hair, found at the scene. Secondly, DNA testing can rule out possible suspects as well. DNA testing can be used to free individuals

Research question What is the effect of temperature Amylase activity? Word count-1453 Background research Enzymes are biological catalysts that speed up a chemical reactions. They do this by decreasing the activation energy(the energy needed to start the reaction) of a chemical reaction. The enzyme present in our saliva is called Amylase. Amylase increases the rate of reaction by decreasing the activation energy needed to hydrolyse the starch molecules.

D Assessment DNA technology Forensic testing 24.11.2014 Marius Martinsen 10D Introduction: I have chosen to investigate Forensic testing, it is also known as DNA profiling or genetic fingerprinting. During this essay I will discuss what the disadvantages and what the advantages of forensic testing are. I will also talk about how forensic testing is carried out. Forensic testing is used to identify an individual by using the DNA sequences of that person.

Finally, the amplified DNA regions are compare using a gel. DNA Profiling