Electrophoresis Lab Report

The STR length contrast is what is used to differentiate individuals. Gel electrophoresis then uses the STRs to create a DNA profile. The gel electrophoresis separates the STRs depending on their length and the pattern is then shown in fluorescent gel creating the profile. These profiles are then used by scientist to compare patterns between evidence and or suspects to determine a match or not a match.

If the catalase enzyme is present in the organism being tested then when in the presence of hydrogen peroxide (H2O2), the enzyme will convert the solution to water and oxygen, this can be observed bubbling of the organism when hydrogen peroxide is added to the test tube. EMB agar is both a selective and differential media; it is selective for gram-negative cells, in that when a gram-positive culture is plated there will be no colonies after incubation because the eosin and methylene dyes prevent the growth of gram-positive organisms, the

First, label one micro centrifuge tube +pGLO and another –pGLO. Using a sterile transfer pipet, transfer 250µl of competent cells (E. coli + CaCl2) into each tube and place them in crushed ice. Examine the pGLO plasmid DNA solution with the UV light and note your observations. Pipet 10µl of pGLO plasmid into the +pGLO tube and mix, close and return it to the ice rack. Do NOT add plasmid DNA to the –pGLO tube.

Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.

DNA Fingerprinting Using Agarose Gel S. Aaron Sowards Bio 122 Lab 04 Brianna Adanitsch Jakob Lester Minhenga Ngijoi 2/21/18 Dr. Chad R. Sethman Abstract DNA fingerprinting is the process of analyzing an individual’s DNA base-pair patterns. The DNA fingerprinting lab involved identifying the suspect using Agarose Gel and Polymerase Chain Reaction. It was found that suspect two s DNA matched the crime scene DNA.

The key enzyme in the process is DNA polymerase that forms the PCR product by linking individual nucleotide bases (adenine, guanine, cytosine and thymine). Short DNA fragments with sequence complimentary to target DNA are called primers; these specify the DNA sequence that has to be amplified. The PCR reaction mixture is made in a 96 well plate or test tube and placed in a thermal cycler. The thermal cycler is a machine used to amplify DNA by altering the temperature in repeated cycles using three precise pre programmed steps: Denaturation – By increasing the temperature in the thermal cycler the DNA is denatured creating separate single stranded DNA

The way plants are able to provide the entire food chain with nucleic acids is that because of the phosphorous and nitrogen cycle, it is able to absorb these two elements and create its own nucleic

DNA samples are submitted to a certified laboratory and undergo the following process (DNA Evidence. n.d.): • Extraction is the process of releasing the DNA from the cell. • Quantitation is the process of determining how much DNA you have. • Amplification is the process of producing multiple copies of the DNA in order to characterize it. • Separation is the process of separating amplified DNA product to permit subsequent identification.

The measurements for Strand A beginning with the length before adding the solution A(ATP only) was 13 mm, after adding the solution the strand contracted to 11mm. The net change was 2mm and the percentage of contraction of the original length was 15%. The measurements for Strand B beginning with the length before adding the solution B(ATP and Salts only) was 7 mm, after adding the solution the strand contracted to 5mm. The net change was 2mm and the percentage of contraction of the original length was 29%. The measurements for Strand C beginning with the length before adding the solution C(Salts only) was 11 mm, after adding the solution the strand contracted to 11mm.

The purpose of this experiment is to measure the concentration of virus in

It is vey important crime investigation feild where the criminal can traced out. It also plays a major role in the law of justice where people lie about their relation that they had with other people(father and son or mother and son). 5.Below are the images of the gels after electrophoresis. Indicate which lane is loaded with the samples from your group?

At each PCR cycle it is possible to measure the amount of amplified product. The detection is performed using non-specific fluorescent dyes that intercalate with any double-stranded DNA or using sequence-specific DNA probes. After each cycle, to estimate the DNA concentration, the fluorescence is measured with a detector and is compared with a control used as reference. Given its capacity to detect the presence and abundance of a specific DNA sequence, RT-PCR techniques have been developed to quantify HPV-DNA in clinical samples (Molijn et al. 2005) and (Dutra et al.

Finally, the amplified DNA regions are compare using a gel. DNA Profiling

DNA and RNA Introduction DNA and RNA are one of the most significant macromolecules in a cell. The transition of information from DNA to RNA and protein determines absolutely all features of a living cell: its size, shape, function and time of death. There are three main sequential mechanisms, by which this transferring of information occurs within a cell: DNA replication, transcription and translation. DNA Replication DNA is a double-stranded macromolecule, which consists of sugar (deoxyribose), phosphate groups, and four types of nucleotides: adenine, thymine, cytosine, and guanine. This molecule also has two different ends: 3’ and 5’; its strands are antiparallel.

Introduction: Forensic dentistry is defined by Keiser-Neilson in 1970 1 as “that branch of forensic medicine which in the interest of justice deals with the proper handling and examination of dental evidence and with the proper evaluation and presentation of the dental findings”. It is application of dental knowledge which helps in legal proceedings. It has an important role in establishing the gender of victims whose bodies are mutilated beyond recognition due to major mass disasters. In the field of forensics experts utilize skeletal remains, features of teeth such as morphology, crown size, root lengths etc. for establishing gender.2 Forensic dentists have a major role in investigations for the identification of people in mass disasters,