In Your Notebooks Determine What Quarter Of The Petri Dish

Introduction The purpose of this Lab was to identify the density of the unidentified object and determine what substance the unidentified object given by the teacher was. The density calculated in the experiment will stay the same because the density of the unidentified object will stay constant. The Independent Variable of this experiment was the calculated density and the unidentified object given. The Dependant Variable for this experiment was the density.

Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.

I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided

Chemistry 1051 Portfolio Over my time in chemistry 1051, I have learned many valuable lessons, and skills. Accurate recording of data was one of those skills, as was creating a well-organized lab write up that correctly laid out the process we completed. During the very first lab we were also given the task of creating our own method to test the density of a peace of glass. We decided to both measure and weigh the glass, effectively testing the density, and afterwards critiquing both methods and choosing the most effective one.

First, label one micro centrifuge tube +pGLO and another –pGLO. Using a sterile transfer pipet, transfer 250µl of competent cells (E. coli + CaCl2) into each tube and place them in crushed ice. Examine the pGLO plasmid DNA solution with the UV light and note your observations. Pipet 10µl of pGLO plasmid into the +pGLO tube and mix, close and return it to the ice rack. Do NOT add plasmid DNA to the –pGLO tube.

Based off the data that was collected, it is safe to say that if the dish contained the pGLO plasmid, the E. Coli growed. If there was no pGLO in the dish, then the only way E. Coli could grow is if it did not contain ampicillin which is the antibiotic. The only way that the E. Coli could grow is if the dish contained ampicillin and arabinose. Since arabinose is essentially and key that turns on the fluorescent glowing, it is essential to have in order to make the bacteria glow. Since no pGLO was present in one dish and ampicillin was also present, the bacteria were unable to grow.

The second step performed was the Gram Stain test. The Gram Stain was used to determine whether the unknown bacterium was gram-positive or gram-negative. First, the unknown sample was smeared onto a slide along with a drop of distilled water. Second, the unknown was air dried and was heat fixed.

Question: Is Mcdonald’s hamburgers safe to eat? Hypothesis: Mcdonald 's hamburger meat contains bacterias that are harmful to the human body. Test the Hypothesis •We can test Mcdonald’s hamburger meat for bacteria. If we find bacterias in the meat we should identify what type of bacteria it is, and what effect does it have on the human body.

In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.

My third hypothesis is that the Member’s Mark will hold the most pennies when it is dry because Member’s Mark is two-ply and appears the thickest. PROCEDURE To prepare for both experiments I will acquire five brands of toilet paper, rubber bands, and a stopwatch from local stores. There is no control group for any of the experiments because I am comparing brands of toilet paper.

The methods of this experiment are really simple. When we started the experiment we all washed our hands and wore gloves. Each group member did their part to conduct and successful experiment, one group member plugs the Bunsen burner to the gas pipe and turned on the gas, then used flint spark lighter to set the flame on the Bunsen burner. While the second group member is setting the dissecting microscope and making sure the lenses are clean. This member is getting all three Petri dishes ready to examine (first Petri dish contains E. coli, the second dish have the mixture of C. elegans, the third dish is where we are transferring female C. elegans to mate up).

The samples of water would need to be cultured on a buffed charcoal yeast medium. The cultures are keep for a minimum of 14 days before the lab can confirm a negative result. I believe that the Legionella bacteria is the reason why 70 workers in the same building became ill around the same time. Proper water treatment standard in the building my have prevented so many workers become ill and avoided the reported death of one

Do the same for the other test tubes. Let the test tubes not be disturbed for about 3- 4 mins. Then add the Amylase solution to the Starch solution and start the stopwatch (immediately). After every 1 min take one drop from the test tubes and place then in the test plate that were

From the Unknown tube professor Cooper gave me, I scratched a little on the slant surface with the sterilized inoculating loop. Then I place it on a clean prepared slide which already had a slight drop of water. The two substances are mixed together in the middle of the slide and let dry completely. One extra step of “heat fix” is necessary to adhere everything to the surface of the slide. To start gram staining, I slightly pour crystal-violet all over the slide and let it sit for 30 seconds before wash it off with water.

Part I Assembly of respirometers: 1. A total of 4 respirometers were set up. The respirometers are numbered 1, 2, 3 and 4. 2. Germinating seeds are placed in Respirometer 1, fresh seeds and beads are in Respirometer 2, dry seeds and beads in respirometer 3 and beads alone are placed in Respirometer 4.