Meat Pies: Bacteria Case Study

Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.

Mannitol Salt Agar (MSA) plate, MacConkey agar (MC) plate, Eosin Methylene Blue agar (EMB), and Hektoen Enteric Agar (HEA) (3). The MacConkey agar plate and the Mannitol Salt agar plate are both used in the identification of the unknown. The MC plate is a selective and differential medium. It is considered a selective medium because the bile salts and crystal violet aspect of the medium prevent the growth of gram positive bacteria (3). This medium is differential because of the lactose and neutral red.

I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided

Winnable Battle- Safe Food Objectives: Approximately 1 of 6 people in America get sick from eating contaminated food. Some people recover and few others may suffer from complication such as kidney failure, miscarriage or brain and nerve damage (10 Colorado winnable battles). In Colorado, there is at least 41% of foodborne outbreaks report and investigation each year. This is an enormous number that underscores the need for vigilance and highlights the importance of the food safety programs to identify foodborne illnesses (10 Colorado winnable battles).

Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.

Readers not only learn why microbes emerge at these particular stages in human civilization and how they cause lethal infections, diseases, and pandemics, but also how microbes, with their dynamic interactions with humans, impact and shape human life. In addition, Crawford tracks human progress towards understanding the cause of communicable diseases and fighting against the disease-causing microbes with treatments and preventions, which includes antibiotics and vaccinations. In the final chapter of Deadly Companions, Crawford outlines our current situation with evolving pathogenic microbes despite the interference from modern advancement and knowledge. These killer microbes continue to exploit society; especially with poverty, travel, and drug behavior providing opportunities for our deadly companions to

Enterobacteriaceae - Enterobacteriacaea is a family of gram-negative, anaerobic, rod-shaped bacteria that are usually motile and consist of saprophytes and parasites of worldwide distribution. They can be found in soil, water, plants and animals. Q2I: Mutation - Mutation is an inheritable change in the base sequence of the genome of an organism.   Question Set 3: Q3A: The authors hypothesized that colistin resistance was spreading by horizontal gene transfer as opposed to mutation.

Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the

The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not

Children under the age of five, the elderly, and people with impaired immune system are the most likely to suffer from illnesses caused by E. coli 0157:H7. The pathogen is now the leading cause of kidney failure among the children in the United States.” The use of logos in Schlosser’s book makes his declaration better because he is using reasonable facts and statistics. Logos makes Schlosser’s claim persuasive and serious. Nowadays, Escherichia coli (E. coli) bacteria is becoming a well-known threat that can be caught by consuming contaminated food (raw vegetables and certain raw meats) or water.

Escherichia Coli 0157: H7 This paper will specialize on a specific type of bacterial foodborne illness caused by the bacteria Escherichia Coli. E. coli was discovered by Theodore von Escherich in 1885. E.coli is a natural found bacteria that lies throughout the intestinal tract of warm blooded animals and comes in many forms only one of which is deadly. This form is E. coli 0157:H7 which can be caused by direct exposure to fecal matter to kill this rouge

The second ½ of the organism was used for gram staining. The gram stain method was performed on the unknown organism per lab manual page 42 and two gram stain reactions were identified. Organism B was gram positive cocci in grape like clusters. Because organism B was positive I could eliminate Escherichia coli, Enterobacter aerogenes and Proteus vulgaris because these bacteria would be rod shapes. Organism A was gram negative pink rod shaped and because of gram positive morphology I could eliminate Staphylococcus aureus, Streptococcus lactis and Bacillus subtilis.

After experiment on microscope under oil immersion, I learned that my Unknown is gram positive. Under the lens, the bacteria appears in purple color. Its morphology is cocci arranged in cluster. However, during decolorizing process, I put too much alcohol to the crystal violet-iodine complex making the color overly removed. That led to the result of my gram positive has slightly redish

INTRODUCTION: Quick look at your hands do you see them. (attention) Do I see what you might be asking? Well the millions of bacteria that are currently hanging out on your hands.

I. There are numerous amounts of diseases all over the world. In the present time, these diseases are cured or contained by vaccines. A couple centuries ago, doctor Edward Jenner developed the first vaccine in 1796. He discovered this vaccine by observing his ambiance. Jenner realized that milkmaids (tend to cattle) frequently contracted cowpox, but after they convalesced they were immune to the deadlier disease smallpox.