All electrochemical measurements were performed with an Autolab potentiostat/galvanostat (PGSTAT 101, Eco Chemie, The Netherlands) interfaced with a Nova 1.11 software. A three-electrode system was used in this work consisting of a platinum wire as the auxiliary electrode, an Ag/AgCl/KCl (saturated) as the reference electrode, and a modified carbon paste electrode as the working electrode. A metrohm 827 pH lab (Herisau, Switzerland) supplied with a combination glass-reference electrode was used for pH measurements.
2.2. Materials
Acetaminophen powder and TiO2 nanoparticles were purchased from Sigma-Aldrich (Taufkirchen, Germany). A 5.0 mM stock solution of acetaminophen was prepared by dissolving an appropriate amount of the powder in a small
…show more content…
Paraffin oil was then added to the mixture and mixed until a uniformly wet paste was achieved and then the resulting paste was placed in the bottom of a glass tube.The electrical connection was established via a copper wire to the paste. The resulting electrode was designated as chitosan/TiO2 nanoparticles-carbon paste electrode (ChiTNP-CPE).The surface of the electrode was gently flattened by rubbing it on a piece of filter paper until it is completely uniform, smooth,and shiny. Finally, it was rinsed with double distilled water. An unmodified carbon paste electrode (CPE) without any contain chitosan and TiO2 nanoparticles was made in a similar way for use in comparative …show more content…
Next, while stirring the solution for 400 sec, a 0.50 V voltage was applied to the electrode. The electrode was then washed thoroughly with sodium acetate-acetic acid buffer solution. The obtained electrode is called ChiTNP-CPE/DNA.To evaluate the DNA immobilization on the electrode surface,the study of cyclic voltammetry[Fe(CN)6]3-/4- redox couple was performed at the presence and absence of DNA.The work is based on the large electrostatic repulsion between this redox couple and the negatively charged DNA [22]. For this purpose, cyclic voltammograms (CVs) of 20 mL of a solution containing 0.05 M [Fe(CN)6]4− and 0.5 M KClwere performed on the surface of bare CPE, ChiTNP-CPE, and ChiTNP-CPE/DNA at a scan rate of 100 mV/s (Fig. 1). As can be seen, a pair of reduction and oxidation peaks is found for the [Fe(CN)6]3-/4- redox couple at CPE surface.At the surface of ChiTNP-CPE, the currents of both peaks increase along with an improvement in the redox reversibility due to electrostatic adsorption of negative species of [Fe(CN)6]3-/4- and cationic polymer in chitosan. As can be seen, immobilization of DNA on the surface of ChiTNP-CPE noticeably decreases the peak currents and redox reversibility of the indicator. This observation suggests that the negatively charged phosphate groups of DNA lead to the electrostatic repulsion of the redox couple on the
This layer is virtually invisible as it has a very small thickness and is also transparent. In order to prevent the fogging and dirt on glasses, the coating must be under UV illumination for it to sustain the hydrophobic property. 3.2.2 Methods to fabricate TiO2-SWCNT composite There are enormous methods available to prepare the nanocomposite of CNT and TiO2 which are sol-gel method, electrospinning method, hydrothermal and more. 3.2.2.1 Sol-gel synthesis Sol-gel is a method in which small molecules are used to produce solid materials. The method is used for the fabrication of metal oxides.
1mL of Acetic acid was then added to Unknown D and the solution was stirred. Next, 15mL of sodium
Figure 20 and 21 casting tray Figure 22 gel box After 1 hr., take out the gel from gel box carefully, place it into the machine, so that the DNA gel electrophoresis can be visualize under UV light. Figure 23 gel documentation system, used to visualize gel electrophoresis with UV light NMR spectroscopy First of all, 3 samples were prepared, peptide in SDS, DPC, and buffer. The sample temperature was maintained at 298 K, prepared by supervisor and H(hydrogen) in SDS and DPC micelle was replaced with D(deuterium) , so that in proton NMR, peptide won’t be interfered by H in micelle. amount of peptide in sds and dpc
The quantitative solubility of the unknown compound was determined to be 29/100ml. The known solubility of sodium sulfate is 28.11g/100mL water. Using the found solubility to compare to the known solubility of sodium sulfate. This solution created in the solubility test, the conductivity of the unknown compound was tested using an Ohmmeter to measure the resistance of the solution. Resistance is the measure of a substances ability to conduct
Lastly, the unknown compound was reacted with two different salts. For the first salt, 0.50 grams of KCl was mixed with 5 mL of water in one beaker while 0.5 grams of NaNO3 was mixed with 5 mL of water in a different beaker. Then, the NaNO3 solution was added to the KCl solution. To perform the reaction with the second salt, 0.50 grams of KCl was mixed with 5 mL of water and 1 mL of 1 M Ag(NO3)2 was added. After performing each reaction, the solution was observed to see if a reaction occurred and the pH value of the resulting solution was tested using a pH
Structural Features Acetaminophen is a molecule that is made of twenty atoms; its bonds consist of fourteen single bonds and four double bonds. Acetaminophen is also composed of clusters atoms with groups with names. First of all, part of an acetaminophen atom is a benzene ring. A benzene ring is a ring of six carbon atoms that are connected to each other by three double bonds and three single bonds and unusually all the bond are the same size. In addition, to make sure all the electrons in the outer valence are used the there are hydrogen atoms bonded to stabilize the carbon atoms.
While conducting this experiment readings were took every 15 seconds for 5 minutes which equals to a total of 20 readings. This experiment had four different trials, a base line trail, a pH 4 trail, a pH of 7 trail, and a pH of 8 trail. Then the results were recorded. The enzyme peroxidase was taken from the inside of a turnip (2g), then blended with 150ml of deionized water, and then poured through a coffee filter into a beaker for a smooth solution. Secondly the colorimeter had to be calibrated by placing a clean cuvette only containing the enzyme solution.
The purpose of this experiment was to understand the pharmacokinetics of the drug acetaminophen within the body, specifically focusing on its partition coefficient, drug protein interaction and its bioavailability through various form of administration. The bioavailability of the drug was determined to be 100% for IV because the drug is injected directly into the systemic circulation in its active form and this is also visible on Figure 4, where the initial concentration of drug is much higher than in PO and IP. For PO and IP administration, the bioavailability was determined to be 72.6% and 39.1%, respectively. This makes sense because both of these type of administration involve the first-pass effect where a portion of the drug is metabolized by peripheral organs, especially the liver in this case, and therefore the amount of active drug reaching the circulation is less. PO administration, however has a much higher content reaching the circulation than IP, because the IP route involves passing through the whole gastrointestinal tract before being absorbed in the liver while the IP route injects the drug into the
Pediatric dosing for Acetaminophen for child < 60 kg is 10 to 15 mg/kg/dose orally every 4-6 hours. A maximum dose of 75 mg/kg/day in infants. This medication is contraindicated if the child has active or severe hepatic disease.
Pure acetaminophen is a white, semi transparent, crystal in the shape of a large monoclinic prism. Acetaminophen is very slightly soluble in cold water but is considerably more soluble in hot water. The recommended dosage of acetaminophen is of 0.5 grams for children from 7-12, taken every 4-6 hours and 1 gram for anyone aged 12-Adult, taken every 4-6 hours. As acetaminophen is a toxic drug an overdose can cause liver damage resulting in death. The reason for this toxicity isn't because of the benzene ring found in acetaminophen, which can also cause liver damage, but rather a metabolite known as N-acetyl-p-benzoquinone imine (NAPQI).
Introduction: The unknown acid molarity will be determining by titration method. Titration is a process depends on concentration of known solution to another solution until the solute in the another solution completely react. Standard solution is the solution of known concentration that used in titration. In this experiment, NaOH was the titrant (base) however, the two analyte which used were HCl and H2SO4.
After reading over the information provided it is clear that ET and the physician have a right to be concerned. It appears as though EF relied on medication a little too much. As a result, it’s likely that she’s suffering from liver disease, which can be caused by an overdose in medications. In order to properly diagnose what was wrong with EF it’s important to look at the ingredients that make up the numerous medications she was consuming. Even more importantly, when looking at the ingredients it’s crucial to keep an eye out for acetaminophen, which can also be written as APAP.
In this lab, we tested 8 known ingredients to find what ingredients was in our unknown A and unknown B medications. We first tested the water solubility of our knowns and unknowns. We found that of the knowns, cornstarch and acetaminophen were the only ones not water soluble. The unknowns were also not water soluble. Th next test was the pH test.
+ -.0005g of KNO3 dissolved in 20 + -0.5 mL. A 10 mL graduated cylinder was used to accurately measure. The pH of potassium nitrate was recorded and the probe was removed and
The main objective of this experiment was the formation of phenacetin from the synthesis of acetaminophen. This was done through a chemical reaction known as the Williamson ether synthesis using techniques of refluxing, vacuum filtration and recrystallization incorporating a mixed solvent system. A further objective of this experiment was to study the formation of the product (phenacetin). Such validation was completed by using techniques for determining the melting point, calculating percent yield, and IR (infrared spectroscopy) of the resultant product.